QUANTITATIVE INTERPRETATION OF BIOMEMBRANE STRUCTURE BY RAMAN-SPECTROSCOPY
QUANTITATIVE INTERPRETATION OF BIOMEMBRANE STRUCTURE BY RAMAN-SPECTROSCOPY
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DOI：
10.1016/0005-2736(77)90078-5
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
PETICOLAS, WL
中科院分区：
文献类型：
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作者：
GABER, BP;PETICOLAS, WL
通过对磷脂光谱结构敏感特征的起源和定量有序参数的发展的研究，扩展了拉曼光谱用于检测生物膜结构的应用。一个参数给出了全反式键在碳氢链中的比例的定量测量，而另一个参数提供了链之间横向类晶体有序度的半定量估计。这些参数被用来研究二棕榈酰磷脂酰胆碱、二肉豆蔻酰胆碱和卵磷脂的囊泡和分散体之间的差异。二棕榈酰磷脂酰胆碱的囊泡在纵向和横向的有序性都大大低于分散体，后者的有序性大大降低。非常仔细地测量了随温度变化的有序性，发现二棕榈酰磷脂酰胆碱的分散体中存在一个预熔转变，而这一转变并不存在于囊泡中。拉曼光谱技术与以前的量热测量和理论计算得到了显著的一致。
The use of Raman spectroscopy to examine the structure of biomembranes is extended through an examination of the origins of the structure-sensitive features of phospholipid spectra and the development of quantitative order-parameters. One parameter gives a quantitative measure of the fraction of all-trans bonds in the hydrocarbon chains while the other provides a semiquantitative estimate of the lateral crystal-like order between the chains. The parameters are used to study the differences between vesicles and dispersions of dipalmitoyl phosphotidylcholine, dimyristoylcholine and egg lecithin. The vesicles of dipalmitoyl phosphotidylcholine are substantially less ordered than the dispersions in terms of both longitudinal and lateral order which are greatly decreased. A very careful measurement of the order as a function of temperature shows that there is a pre-melting transition in the dispersions of dipalmitoyl phosphotidylcholine which does not exist in the vesicles. Remarkable agreement was obtained between the Raman technique and that previously reported by calorimetric measurements and theoretical calculations.