Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis
Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis
复制标题
DOI：
10.1104/pp.004416
复制
发表时间：
2002-09-01
期刊：
影响因子：
7.4
通讯作者：
Hauser, MT
中科院分区：
文献类型：
--
作者：
Müller, S;Fuchs, E;Hauser, MT
在筛选拟南芥根形态发生调控因子时，我们分离到了6个细胞扩增不规则的隐性突变体。互补分析将突变定位在PLEIADE （PLE）和HYADE （HYA）两个位点上。表型分析显示，在不完全分离的细胞中存在多核细胞、细胞壁残根和同步细胞分裂，这些都是细胞分裂缺陷的特征。这些缺陷在根部明显，而在空气器官中无法检测到。此外，育性和发芽率不受突变的影响。因此，我们从PLE和HYA中分离出的等位基因表明，这些基因可能编码了根发育过程中主要需要的器官特异性成分。对单根和芽根细胞周期微管排列的分析表明，几个同步核的存在影响了前期带、有丝分裂纺锤体和膜质体的位置。PLE/ PLE的增强型和增效型。hya/hya幼苗和双突变体表明了PLE和hya在同一过程中的作用。这些突变体为阐明细胞分裂和细胞质分裂过程中核细胞骨架相互作用的调控提供了工具。
In screens for regulators of root morphogenesis in Arabidopsis we isolated six new recessive mutants with irregular cell expansion. Complementation analyses placed the mutations in two loci, PLEIADE (PLE) and HYADE (HYA). Phenotypic analyses revealed multinucleated cells, cell wall stubs, and synchronized cell divisions in incompletely separated cells that are all characteristics of defective cytokinesis. These defects were pronounced in roots and undetectable in aerial organs. In addition, fertility and germination were not affected by the mutations. Thus, the alleles that we have isolated of PLE and HYA suggest that the genes may encode organ-specific components needed primarily during root development. Analysis of microtubule arrays during cell cycle in ple and hya roots indicates that the presence of several synchronized nuclei influences the position of preprophase band, mitotic spindles, and phragmoplasts. The enhanced and synergistic phenotype of PLE/ple.hya/hya seedlings and double mutants point to a role of PLE and HYA in the same process. These mutants provide tools to elucidate the regulation of nuclear cytoskeletal interactions during cell division and cytokinesis.