In Vivo Imaging Using Quantum Dot–Conjugated Probes
In Vivo Imaging Using Quantum Dot–Conjugated Probes
复制标题
使用量子点共轭探针进行体内成像
DOI：
10.1002/0471143030.cb2501s36
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发表时间：
2007
影响因子：
--
通讯作者：
Donna J Arndt
中科院分区：
文献类型：
--
作者：
Diane S Lidke;P. Nagy;Donna J Arndt
本单元描述了量子点（QD）用于活细胞成像以及在流式细胞术中使用QD定量分析配体结合常数和受体密度。传统的荧光团和可见荧光蛋白（VFP）的结构已经允许许多细胞过程的可视化。然而，有机和生物分子荧光团在其应用中具有限制，这是由于它们的小斯托克斯位移和在长时间成像期间的光漂白倾向。与传统荧光团相比，量子点具有许多优势，包括高亮度和光稳定性，这使它们成为活细胞成像的特殊工具。存在各种各样的具有不同表面反应性和特性的市售QD。作者将此处提供的实验室方案限制为使用链霉亲和素偶联的QD，因为这通过将识别受体的配体或抗体偶联至生物素并通过使用QD可视化复合物而几乎普遍适用于任何细胞表面受体。Curr.方案Cell Biol.36：25.1.1 - 25.1.18.© 2007年由约翰威利父子公司。
This unit describes the use of quantum dots (QDs) for live‐cell imaging and the use of QDs in flow cytometry for quantitative analysis of ligand binding constants and receptor density. Conventional fluorophores and visible fluorescent protein (VFP) constructs have allowed visualization of many cellular processes. However, organic and biomolecular fluorophores have limitations in their applications, due to their small Stokes' shift and tendency to photobleach during prolonged imaging. QDs have many advantages over conventional fluorophores, including high brightness and photostability, which make them an exceptional tool for live‐cell imaging. There are a large variety of commercially available QDs with different surface reactivities and characteristics. The authors have limited the laboratory protocols presented here to the use of streptavidin‐coupled QDs because this gives almost universal applicability to any cell surface receptor by coupling the ligand or antibody that recognizes the receptor to biotin and visualizing the complex by use of QDs. Curr. Protoc. Cell Biol. 36:25.1.1‐25.1.18. © 2007 by John Wiley & Sons, Inc.
影响因子：
1.1
作者：
Ivan Martin;Bojana Obradovic;S. Treppo;A. Grodzinsky;R. Langer;L. Freed;G. Vunjak‐Novakovic
通讯作者：
Ivan Martin;Bojana Obradovic;S. Treppo;A. Grodzinsky;R. Langer;L. Freed;G. Vunjak‐Novakovic