Identification of Human Very Small Embryonic like Stem Cells (VSELS) in Human Heart Tissue Among Young and Old Individuals
Identification of Human Very Small Embryonic like Stem Cells (VSELS) in Human Heart Tissue Among Young and Old Individuals
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DOI：
10.1007/s12015-019-09923-1
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发表时间：
2019-11-22
影响因子：
4.8
通讯作者：
Abdel-Latif, Ahmed
中科院分区：
文献类型：
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作者：
El-Helw, Mohamed;Chelvarajan, Lakshman;Abdel-Latif, Ahmed
极小胚胎样干细胞(VSEL)是一种多能干细胞，存在于成人组织中。VSEL存在于多种组织中，包括骨髓、脐带血和性腺。它们表现出胚胎干细胞的多种特征，包括分化为包括心肌细胞和血管内皮细胞在内的所有三个生殖层的细胞谱系的能力。然而，它们在成人的固体器官中的存在，如人类的心脏，还没有得到证实。VSEL是有价值的干细胞来源，用于组织再生和用于周转和常见磨损的细胞替代。本研究的目的是探索人类心脏组织中是否存在人类VSEL(HuVSEL)，并检测其患病率随年龄和心脏病的变化。从健康和缺血性心脏病受试者采集的人心脏组织检测VSEL的患病率，定义为CD45-/CD133+/SSEA4+。对心外膜和心内膜组织进行检查，比较不同年龄组的VSEL数量。我们的数据证实了huVSEL在成年人心脏中的存在，随着年龄的增长，患病率下降。这是首次在成人心脏组织中发现huVSEL的证据。心脏huVSEL可以在未来的研究中进一步探索，以表征它们在再生研究中的原始潜力和治疗潜力。
Very Small Embryonic-Like (VSEL) stem cells are a proposed pluripotent population, residing in adult tissues. VSELs have been described in multiple tissues including bone marrow, cord blood, and gonads. They exhibit multiple characteristics of embryonic stem cells including the ability to differentiate into cellular lineages of all three germ layers, including cardiomyocytes and vascular endothelial cells. However, their presence in adult solid organs such as heart in humans has not been established. VSELs are valuable source of stem cells for tissue regeneration and replacement of cells for turnover and usual wear-and-tear. The purpose of our study was to explore the existence of human VSELs (huVSELs) in human heart tissue and examine the changes in their prevalence with aging and cardiac disease. Human heart tissue, collected from healthy and ischemic heart disease subjects was examined for the prevalence of VSELS, defined as CD45-/CD133+/SSEA4+. Both epicardial and endocardial tissues were examined comparing VSEL numbers across different age groups. Our data confirm the existence of huVSELs in adult hearts with decreasing prevalence during aging. This is the first evidence of huVSELs in adult cardiac tissue. Cardiac huVSELs could be further explored in future studies to characterize their primitive potential and therapeutic potential in regenerative studies.