Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis
Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis
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DOI：
10.1007/978-981-13-0502-3_8
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发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Nguyen, Cuong Q.
中科院分区：
文献类型：
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作者：
Voigt, Alexandria;Semenova, Touyana;Nguyen, Cuong Q.
自1975年Kohler和Milstein发现小鼠杂交瘤技术以来，单克隆抗体生产取得了重大进展。 B 细胞永生化和噬菌体展示技术的进步已经产生了大量用于诊断和治疗的有价值的单克隆抗体。 Technological breakthroughs in various fields of 'omics have shed crucial insights into cellular heterogeneity of a biological system in which the functional individuality of a single cell must be considered.基于这一重要概念，单细胞分析在识别和分离产生有益的治疗性单克隆抗体的功能性 B 细胞方面取得了显着的发现。在这篇综述中，我们将讨论抗体发现的三种传统方法。将回顾最近用于单细胞抗体发现的技术平台。我们将讨论单细胞分析在寻找人类免疫缺陷病毒和新出现的寨卡虫媒病毒的治疗抗体中的应用。
Since the discovery of mouse hybridoma technology by Kohler and Milstein in 1975, significant progress has been made in monoclonal antibody production. Advances in B cell immortalization and phage display technologies have generated a myriad of valuable monoclonal antibodies for diagnosis and treatment. Technological breakthroughs in various fields of 'omics have shed crucial insights into cellular heterogeneity of a biological system in which the functional individuality of a single cell must be considered. Based on this important concept, remarkable discoveries in single-cell analysis have made in identifying and isolating functional B cells that produce beneficial therapeutic monoclonal antibodies. In this review, we will discuss three traditional methods of antibody discovery. Recent technological platforms for single-cell antibody discovery will be reviewed. We will discuss the application of the single-cell analysis in finding therapeutic antibodies for human immunodeficiency virus and emerging Zika arbovirus.