Echinococcus multilocularis laminated-layer components and the E14t 14-3-3 recombinant protein decrease NO production by activated rat macrophages in vitro
Echinococcus multilocularis laminated-layer components and the E14t 14-3-3 recombinant protein decrease NO production by activated rat macrophages in vitro
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DOI：
10.1016/j.niox.2004.03.002
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发表时间：
2004-05-01
影响因子：
3.9
通讯作者：
Muro, A
中科院分区：
文献类型：
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作者：
Andrade, MA;Siles-Lucas, M;Muro, A
多叶棘球绦虫和细粒棘球绦虫分别在人和动物中引起泡状和囊状(单眼)包虫病。众所周知，这些寄生虫可以影响周围寄生宿主细胞产生的一氧化氮(NO)等分子。然而，到目前为止，还没有对影响细胞NO产生的寄生虫成分进行详细的剖析。我们比较了细粒棘球绦虫和多房棘球绦虫限定的后囊线虫结构成分(层状层相关)和代谢成分(14-3-3蛋白，可能与多房棘球绦虫肿瘤样生长相关)对体外培养的大鼠肺泡巨噬细胞产生NO的影响。结果表明，这些抗原在体外均不能刺激巨噬细胞产生NO。然而，当细胞预先暴露于脂多糖刺激时，某些棘球绦虫抗原在体外对NO产生的影响被逆转。当使用这两个物种的多房棘球菌层压层(LL)或囊壁(CW)可溶性成分时，这种抑制作用被发现。在细胞与E14t蛋白孵育后，用内毒素预刺激细胞后，NO和iNOS mRNA的产生也明显减少，且呈剂量依赖关系。因此，多房棘球绦虫14-3-3蛋白似乎是CW和LL元线虫提取物抑制作用的成分之一。(C)2004 Elsevier Inc.保留所有权利。
Echinococcus multiloeularis and Echinococcus granulosus cause alveolar and cystic (unilocular) echinococcosis, respectively, in humans and animals. It is known that these parasites can affect, among other molecules, nitric oxide (NO) production by periparasitic host cells. Nevertheless, detailed dissection of parasite components specifically affecting cell NO production has not been done to date. We compare the effect of E. granulosus and E. multilocularis defined metacestode structural (laminated-layer associated) and metabolic (14-3-3 protein, potentially related with E. multilocularis metacestode tumor-like growth) components on the NO production by rat alveolar macrophages in vitro. Our results showed that none of these antigens could stimulate macrophage NO production in vitro. However, a reversed effect of some Echinococcus antigens on NO in vitro production was found when cells were previously exposed to LPS stimulation. This inhibitory effect was found when E. multilocularis laminated-layer (LL) or cyst wall (CW) soluble components from both species were used. Pre-stimulation of cells with LPS also resulted in a strong, dose-dependent reduction of NO and iNOS mRNA production after incubation of cells with the E14t protein. Thus, the E. multilocularis 14-3-3 protein appears to be one of the components accounting for the suppressive effect of the CW and LL metacestode extracts. (C) 2004 Elsevier Inc. All rights reserved.