Icariin Accelerates Fracture Healing via Activation of the WNT1/β-catenin Osteogenic Signaling Pathway
Icariin Accelerates Fracture Healing via Activation of the WNT1/β-catenin Osteogenic Signaling Pathway
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DOI：
10.2174/1389201021666200611121539
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Xu, Can-Hong
中科院分区：
文献类型：
--
作者：
Zhang, Xiao-Yun;Chen, Yue-Ping;Xu, Can-Hong
Background: Icariin has been shown to enhance bone formation. Objective: The present study aimed to investigate whether icariin also promotes bone fracture healing and its mechanisms.Methods: First, we isolated and cultured rat bone marrow stromal cells (rBMSCs) with icariin-containing serum at various concentrations (0%, 2.5%, 5% and 10%) and then measured alkaline phosphatase (ALP) activity and the expression of Core-binding factor, alpha 1 (Cbf alpha 1), bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein-4 (BMP-4) in the rBMSCs. Second, we established a model of fracture healing in rats and performed gavage treatment for 20 days. Then, we detected bone biochemical markers (ELISA kits) in the serum, fracture healing (digital radiography, DR), and osteocalcin expression (immunohistochemistry).Results: Icariin treatment increased ALP activity and induced the expression of Cbf alpha 1, BMP- 2 and BMP-4 in rBMSCs in a dose-dependent manner. In addition, Icariin increased the serum levels of osteocalcin (OC), bone-specific alkaline phosphatase (BAP), N-terminal telopeptides of type I collagen (NTX-1), C-terminal telopeptide of type I collagen (CTX-1) and tartrate-resistant acid phosphatase 5b (TRACP-5b); promoted osteocalcin secretion at the fracture site; and accelerated fracture healing.Conclusion: Icariin can promote the levels of bone-formation markers, accelerate fracture healing, and activate the WNT1/beta-catenin osteogenic signaling pathway.
