Biological Cell Detachment from Poly(N-isopropyl acrylamide) and Its Applications
Biological Cell Detachment from Poly(N-isopropyl acrylamide) and Its Applications
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DOI：
10.1021/la902587p
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发表时间：
2010-06-01
期刊：
影响因子：
3.9
通讯作者：
Canavan, Heather E.
中科院分区：
文献类型：
--
作者：
Cooperstein, Marta A.;Canavan, Heather E.
在过去的二十年中，聚（N-异丙基丙烯酰胺）（pNIPAM）已广泛用于生物工程应用。特别地，pN IPAM基底已被用于生物细胞和蛋白质的非破坏性释放。在这篇专题文章中，我们回顾了pNIPAM底物用于释放生物细胞的应用，包括用于细胞外基质（ECM）的研究，用于细胞片工程和组织移植，形成肿瘤样球体，生物粘附和生物吸附的研究。以及单个细胞的操纵或变形。这些文章包括来自我们自己的小组以及在世界各地进行研究的人的意见书。
Over the past two decades, poly(N-isopropyl acrylamide) (pNIPAM) has become widely used for bioengineering applications. In particular, pN IPAM substrates have been used for the nondestructive release of biological cells and proteins. In this feature article, we review the applications for which pNIPAM substrates have been used to release biological cells, including for the study of the extracellular matrix (ECM), for cell sheet engineering and tissue transplantation, the formation of tumorlike spheroids, the study of bioadhesion and bioadsorption. and the manipulation or deformation of individual cells. The articles reviewed include submissions from our own group as well as from those performing research in the field worldwide.