The Mechanisms of Virulence Regulation by Small Noncoding RNAs in Low GC Gram-Positive Pathogens.
The Mechanisms of Virulence Regulation by Small Noncoding RNAs in Low GC Gram-Positive Pathogens.
复制标题
DOI：
10.3390/ijms161226194
复制
发表时间：
2015-12-14
影响因子：
5.6
通讯作者：
Cho KH
中科院分区：
文献类型：
--
作者：
Pitman S;Cho KH
最近，在细菌中发现的小的非编码调控RNA(SRNAs)得到了巨大的增长，为基因调控提供了新的见解。计算分析和RNA测序的实现为发现和分析潜在的sRNA提供了新的工具。通过碱基配对作用于靶向mRNAs的小调控RNA被发现普遍存在，是细菌中转录后调控因子中含量最丰富的一类。大多数sRNA研究仅限于大肠杆菌和其他革兰氏阴性细菌。然而，在革兰氏阳性细菌中发现sRNA的例子仍然很多，尽管它们背后的详细基因调控机制还没有被很好地理解。严格的毒力控制对病原体的生存至关重要，已发现许多sRNA参与了这一过程。该综述概述了参与各种革兰氏阳性病原体毒力调节的反式作用sRNAs的靶点和目前已知的机制。此外，还描述了它们的共同特征，如CU相互作用基序、HfQ的作用以及参与双组分调节、核糖开关、群体感应或毒素/抗毒素系统。
The discovery of small noncoding regulatory RNAs (sRNAs) in bacteria has grown tremendously recently, giving new insights into gene regulation. The implementation of computational analysis and RNA sequencing has provided new tools to discover and analyze potential sRNAs. Small regulatory RNAs that act by base-pairing to target mRNAs have been found to be ubiquitous and are the most abundant class of post-transcriptional regulators in bacteria. The majority of sRNA studies has been limited to E. coli and other gram-negative bacteria. However, examples of sRNAs in gram-positive bacteria are still plentiful although the detailed gene regulation mechanisms behind them are not as well understood. Strict virulence control is critical for a pathogen’s survival and many sRNAs have been found to be involved in that process. This review outlines the targets and currently known mechanisms of trans-acting sRNAs involved in virulence regulation in various gram-positive pathogens. In addition, their shared characteristics such as CU interaction motifs, the role of Hfq, and involvement in two-component regulators, riboswitches, quorum sensing, or toxin/antitoxin systems are described.