Histocompatible miniature swine: An inbred large-animal model
Histocompatible miniature swine: An inbred large-animal model
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DOI：
10.1097/01.tp.0000054839.43852.bf
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发表时间：
2003-03-27
期刊：
影响因子：
6.2
通讯作者：
Sachs, DH
中科院分区：
文献类型：
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作者：
Mezrich, JD;Haller, GW;Sachs, DH
作者的实验室报告了三个小型猪群，每个小型猪群在主要组织相容性复合体（MHC）上具有不同的等位基因组，以及五个MHC内重组株。选择一个牛群（SLA（dd））进行进一步近交，以获得组织相容性品系。它已经经历了连续的兄弟姐妹或父女交配（称为G7）的七个额外的世代。为了确定这些动物的组织相容性水平，作者在没有免疫抑制的情况下进行了皮肤和心脏移植。与在11天内排斥皮肤（中位存活时间[MST]，n=6）和在35天内排斥心脏（MST，n=4）的MHC匹配的、轻微抗原错配的动物相反，G7动物接受皮肤超过340天（>340、>448和>677天）和心脏超过265天（>265和>269天）。尽管如此，第二次移植排斥反应可由致敏诱导，表明弱次要抗原仍然存在，需要进一步近亲繁殖以实现完全的组织相容性。我们得出结论，G7动物是足够的近亲繁殖，接受第一组皮肤和心脏移植无限期。
Three herds of miniature swine, each homozygous for a different set of alleles at the major histocompatibility complex (MHC), and five intra-MHC recombinant strains, have been reported by the authors' laboratory. One herd (SLA(dd)) was selected for further inbreeding to achieve a histocompatible line. It has undergone seven additional generations of sequential brother-sister or father-daughter matings (termed G7). To determine the level of histocompatibility of these animals, the authors performed skin and heart transplantation without immunosuppression. In contrast to MHC-matched, minor antigen-mismatched animals that rejected skin in 11 days (median survival time [MST], n=6) and hearts in 35 days (MST, n=4), G7 animals accepted skin for greater than 340 days (>340, >448, and >677 days) and hearts for greater than 265 days (>265 and >269 days). Nevertheless, rejection of second grafts could be induced by sensitization, indicating that weak minor antigens remain, requiring further inbreeding to achieve full histocompatibility. We conclude that G7 animals are sufficiently inbred to accept first set skin and heart grafts indefinitely.