Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis
Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis
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DOI：
10.1016/j.ydbio.2005.07.013
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发表时间：
2005-10-01
影响因子：
2.7
通讯作者：
Heath, JK
中科院分区：
文献类型：
--
作者：
Ng, ANY;de Jong-Curtain, TA;Heath, JK
最近对一种新型转基因斑马鱼（gutGFP）的分析提供了肝脏和胰腺发育过程中发生的早期形态学事件的详细描述。在本文中，我们的目的是补充这些研究提供所有的形态学事件，形成斑马鱼肠上皮细胞的分析。我们的目标之一是提供一个框架，为未来表征斑马鱼突变表型，其中肠上皮形态发生已被破坏。我们的分析涵盖了受精后26至126小时（hpf）的时间段，并遵循内胚层的连续层的生长，管腔形成和分化为功能性肠上皮细胞，具有三个形态上不同的部分：肠球，中肠和后肠。在26 hpf和76 hpf之间，整个肠内胚层是高度增殖的器官。为了形成管腔，斑马鱼内胚层细胞经历顶膜生物发生，采用双层结构并形成小腔，其在没有细胞死亡的情况下合并。此后，内胚层细胞增殖并分化成不同的细胞谱系。在一个新的稳定的转基因品系Tg[nkx2.2a：mEGFP]中，肠内分泌细胞首先在52 hpf时在肠的尾部区域中被区分。含粘蛋白的杯状细胞的分化在100 hpf时首先明显，并严格限制在肠的中段，称为肠中段，其也通过具有大的核上空泡的肠细胞的存在来划分。同时，吻端肠腔显著扩张，形成肠球。在这里，上皮发达的褶皱和增殖的细胞变得越来越局限于基底室，类似于哺乳动物的Lieberkuhn隐窝。在126 hpf时，后肠仍然是单层未折叠的单层柱状上皮。(c)2005年爱思唯尔公司All rights reserved.
Recent analysis of a novel strain of transgenic zebrafish (gutGFP) has provided a detailed description of the early morphological events that occur during the development of the liver and pancreas. In this paper, we aim to complement these studies by providing ail analysis of the morphological events that shape the zebrafish intestinal epithelium. One of our goals is to provide a framework for the future characterization of zebrafish mutant phenotypes in which intestinal epithelial morphogenesis has been disrupted. Our analysis encompasses the period between 26 and 126 h post-fertilization (hpf) and follows the growth, lumen formation and differentiation of a continuous layer of endoderm into a functional intestinal epithelium with three morphologically distinct segments: the intestinal bulb, mid-intestine and posterior intestine. Between 26 hpf and 76 hpf, the entire intestinal endoderm is a highly proliferative organ. To make a lumen, the zebrafish endoderm cells undergo apical membrane biogenesis, adopt a bilayer configuration and form small cavities that coalesce without cell death. Thereafter, the endoderm cells polarize and differentiate into distinct cell lineages. Enteroendocrine cells are distinguished first at 52 hpf in the caudal region of the intestine in a new stable transgenic line, Tg[nkx2.2a:mEGFP]. The differentiation of mucin-containing goblet cells is first evident at 100 hpf and is tightly restricted to a middle segment of the intestine, designated the mid-intestine, that is also demarcated by the presence of enterocytes with large supranuclear vacuoles. Meanwhile, striking expansion of the lumen in the rostral intestine forms the intestinal bulb. Here the epithelium elaborates folds and proliferating cells become progressively restricted to a basal compartment analogous to the crypts of Lieberkuhn in mammals. At 126 hpf, the posterior intestine remains an unfolded monolayer of simple columnar epithelium. (c) 2005 Elsevier Inc. All rights reserved.