RNA-mediated interference and reverse transcription control the persistence of RNA viruses in the insect model Drosophila
RNA-mediated interference and reverse transcription control the persistence of RNA viruses in the insect model Drosophila
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DOI：
10.1038/ni.2542
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发表时间：
2013-04-01
影响因子：
30.5
通讯作者：
Saleh, Maria-Carla
中科院分区：
文献类型：
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作者：
Goic, Bertsy;Vodovar, Nicolas;Saleh, Maria-Carla
持续性病毒感染是如何建立和维持的，这一点存在广泛的争论，人们仍然知之甚少。我们发现，RNA病毒在果蝇体内的持续存在是通过细胞逆转录酶活性和RNA介导的干扰（RNAi）途径的联合作用实现的。不同RNA病毒的片段在感染早期被逆转录，这导致嵌入逆转录转座子序列中的DNA形式。这些病毒-逆转录转座子DNA嵌合体产生由RNAi机制处理的转录物，这反过来又抑制了病毒复制。相反，逆转录的抑制阻碍了嵌合DNA的出现，并防止持久性。我们的研究结果确定了逆转录转座子和抗病毒RNAi在控制致命的急性感染建立病毒持久性的合作功能。
How persistent viral infections are established and maintained is widely debated and remains poorly understood. We found here that the persistence of RNA viruses in Drosophila melanogaster was achieved through the combined action of cellular reverse-transcriptase activity and the RNA-mediated interference (RNAi) pathway. Fragments of diverse RNA viruses were reverse-transcribed early during infection, which resulted in DNA forms embedded in retrotransposon sequences. Those virus-retrotransposon DNA chimeras produced transcripts processed by the RNAi machinery, which in turn inhibited viral replication. Conversely, inhibition of reverse transcription hindered the appearance of chimeric DNA and prevented persistence. Our results identify a cooperative function for retrotransposons and antiviral RNAi in the control of lethal acute infection for the establishment of viral persistence.