Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability
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DOI：
10.1016/j.bjm.2016.01.002
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发表时间：
2016-06-01
影响因子：
2.2
通讯作者：
Villalba, Laura Lidia
中科院分区：
文献类型：
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作者：
Fonseca, María Isabel;Tejerina, Marcos Raúl;Villalba, Laura Lidia
由白色腐真菌分泌的氧化酶可应用于造纸工业、生物燃料生产和生物修复中的若干技术过程。来自生物多样性米西奥内斯（阿根廷）森林的天然菌株的发现可以为生物技术目的提供有用的酶。在这项工作中，我们评估的漆酶和锰过氧化物酶分泌能力的四个新发现的菌株栓菌属是本地的米西奥内斯。此外，铜的响应和最适pH和温度的培养上清液中的漆酶活性进行了测定。选择的菌株产生的漆酶和MnP的变量;当添加Cu 2+，这两种酶显着增加。酶谱显示，在Cu 2+存在下，所有菌株的两种同工酶均增加。菌株B表现出最大的响应Cu 2+的添加，而菌株A是更稳定的最佳温度和pH值。菌株A显示出有趣的潜力，未来的生物技术方法，由于其分泌的酶的上级热稳定性。(C)2016年巴西微生物学会。出版社：Elsevier Editora Ltda
Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.