The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability.
The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability.
复制标题
人类中期染色体的 24 种荧光模式 - 区分特征和变异性。
作者：
T. Caspersson;G. Lomakka;L. Zech
某些荧光DNA结合化合物，其中包括奎纳克林芥子气和奎纳克林，在人类中期染色体中产生特征性带型;这些模式可用于识别所有24种染色体类型以及染色体畸变。奎纳克林芥子气所产生的模式特别清晰和稳定，适用于染色体鉴定，无论是视觉上的，最好是在对比度增强后，通过荧光光度法。描述了每种染色体类型的典型荧光模式。
的图案的可重复性和可变性进行了分析，从14个健康受试者的约5000条染色体的材料中的图案的光度测量。除了具有特别强的荧光的某些较小但明确定义的染色体区域（其受到某些个体变化的影响）之外，荧光模式显示出相当稳定和可再现。
Certain fluorescent DNA-binding compounds, among them quinacrine mustard and quinacrine, give characteristic banding patterns in human metaphase chromosomes; these patterns can be used to identify all the 24 chromosome types as well as chromosome aberrations. The patterns given by quinacrine mustard are especially clear and stable and are suitable for chromosome identification either visually—preferably after contrast enhancement by photography—or by photometric methods. The typical fluorescence pattern of each chromosome type is described.
The reproducibility and variability of the patterns have been analysed by photometric measurements of the patterns in a material of about 5000 chromosomes from 14 healthy subjects. Apart from certain minor but well defined chromosome regions with especially strong fluorescence, which are subject to certain individual variations, the fluorescence patterns were shown to be quite stable and reproducible.