Improving the viability of tissue‐resident stem cells using an organ‐preservation solution
Improving the viability of tissue‐resident stem cells using an organ‐preservation solution
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使用器官保存溶液提高组织驻留干细胞的活力
DOI：
10.1002/2211-5463.12748
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发表时间：
2019
期刊：
影响因子：
2.6
通讯作者：
Kubo Hiroshi
中科院分区：
文献类型：
--
作者：
Suzuki Takaya;Ota Chiharu;Fujino Naoya;Tando Yukiko;Suzuki Satoshi;Yamada Mitsuhiro;Kondo Takashi;Okada Yoshinori;Kubo Hiroshi
人类临床标本是组织驻留干细胞的宝贵来源，但需要在组织采集后立即采集此类细胞。为了延长从新鲜人体样本中采集的时间尺度，我们开发了一种新的细胞外液（ECF）型保存溶液，该溶液基于含有低分子量葡聚糖和葡萄糖的高钠低钾溶液，用于保存移植器官。在这项研究中，我们比较了使用我们的ECF溶液与使用其他三种溶液（PBS、Dulbecco改良Eagle培养基和Euro柯林斯溶液）保存组织驻留干细胞的效果。这些溶液分别代表通用缓冲液、通用培养基和基准器官保存溶液。从小鼠中取出肺组织，并在低温条件下保存72小时。在测试的溶液中，仅在ECF型溶液中保存可以保持小鼠肺组织驻留干细胞的增殖和分化能力。此外，ECF溶液在4 °C下储存超过7天时可以保持人肺泡上皮祖细胞的活力和增殖。人肺泡II型细胞在低温保存2、5、8和14天时的平均存活率分别为90.9%、84.8%、85.7%和66.3%，8天内无显著差异。总的来说，我们的研究结果表明，使用我们的ECF型保存液可以保持组织驻留干细胞的活力和功能。使用这种保存溶液可以促进目前无法获得的人类干细胞生物学人类组织标本的研究。
Human clinical specimens are a valuable source of tissue‐resident stem cells, but such cells need to be collected immediately after tissue collection. To extend the timescale for collection from fresh human samples, we developed a new extracellular fluid (ECF)‐type preservation solution based on a high‐sodium and low‐potassium solution containing low‐molecular‐weight dextran and glucose, which is used for preservation of organs for transplantation. In this study, we compared the preservation of tissue‐resident stem cells using our ECF solution with that using three other solutions: PBS, Dulbecco’s modified Eagle’s medium and Euro‐Collins solution. These solutions represent a common buffer, a common culture medium and a benchmark organ‐preservation solution, respectively. Lung tissues were removed from mice and preserved for 72 h under low‐temperature conditions. Of the solutions tested, only preservation in the ECF‐type solution could maintain the proliferation and differentiation capacity of mouse lung tissue‐resident stem cells. In addition, the ECF solution could preserve the viability and proliferation of human alveolar epithelial progenitor cells when stored for more than 7 days at 4 °C. The mean viability of human alveolar type II cells at 2, 5, 8 and 14 days of low‐temperature preservation was 90.9%, 84.8%, 85.7% and 66.3%, respectively, with no significant differences up to 8 days. Overall, our findings show that use of our ECF‐type preservation solution may maintain the viability and function of tissue‐resident stem cells. Use of this preservation solution may facilitate the investigation of currently unobtainable human tissue specimens for human stem cell biology.