Do morphogen gradients arise by diffusion?
Do morphogen gradients arise by diffusion?
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DOI：
10.1016/s1534-5807(02)00179-x
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发表时间：
2002-06-01
影响因子：
11.8
通讯作者：
Wan, FYM
中科院分区：
文献类型：
--
作者：
Lander, AD;Nie, Q;Wan, FYM
在发育过程中，细胞和组织组织的许多模式都是由形态发生素的梯度决定的，形态发生素是一种在不同浓度下分配不同细胞命运的物质。形态发生素从局部位点通过形态发生素运输形成，但这是通过简单的扩散还是通过更复杂的机制发生尚不清楚。我们试图通过分析最近的数据来解决这一争议，这些数据以适当的方式捕捉系统的复杂性，在这些系统中，运输，受体相互作用，内分泌和胞吐作用以及降解一起发生。我们发现，扩散机制的形态运输是更易理解的和非扩散机制更不容易理解的，比一般认为。此外，我们表明，一类实验，内吞阻滞，认为有效区分扩散和非扩散运输模型实际上未能得出有用的区别。
Many patterns of cell and tissue organization are specified during development by gradients of morphogens, substances that assign different cell fates at different concentrations. Gradients form by morphogen transport from a localized site, but whether this occurs by simple diffusion or by more elaborate mechanisms is unclear. We attempt to resolve this controversy by analyzing recent data in ways that appropriately capture the complexity of systems in which transport, receptor interaction, endo- and exocytosis, and degradation occur together. We find that diffusive mechanisms of morphogen transport are much more plausible-and nondiffusive mechanisms much less plausible-than has generally been argued. Moreover, we show that a class of experiments, endocytic blockade, thought to effectively distinguish between diffusive and nondiffusive transport models actually fails to draw useful distinctions.