Pex17p is required for import of both peroxisome membrane and lumenal proteins and interacts with Pex19p and the peroxisome targeting signal-receptor docking complex in Pichia pastoris.
Pex17p is required for import of both peroxisome membrane and lumenal proteins and interacts with Pex19p and the peroxisome targeting signal-receptor docking complex in Pichia pastoris.
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Pex17p 是过氧化物酶体膜和腔蛋白输入所必需的，并与毕赤酵母中的 Pex19p 和过氧化物酶体靶向信号受体对接复合物相互作用。
DOI：
10.1091/mbc.10.12.4005
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发表时间：
1999
影响因子：
3.3
通讯作者：
Subramani,S
中科院分区：
文献类型：
--
作者：
Snyder,WB;Koller,A;Choy,AJ;Johnson,MA;Cregg,JM;Rangell,L;Keller,GA;Subramani,S
通过对一株过氧化物酶体膜蛋白（PMP）定位突变株的筛选，获得了一株过氧化物酶体缺陷型毕赤酵母（Pichia pastoris）PEX17，PEX17编码267个氨基酸，与酵母（Saccharomyces pastoris）Pex17p的同源性为18%。与ScPex17 p一样，PpPex17 p在氨基末端附近含有一个假定的跨膜结构域和两个羧基末端卷曲螺旋区。PpPex17 p作为一个完整的PMP与胞浆羧基末端结构域。pex17 Δ突变体积累过氧化物酶体基质蛋白和某些完整的PMP在胞浆中，这表明Pex17 p在其定位的关键作用。通过形态学和生物化学手段观察到Pex17 Δ突变体中存在过氧化物酶体残基，表明Pex17 p不是残基形成的绝对必需条件。酵母双杂交分析表明，Pex19p的羧基末端需要与Pex17 p缺乏羧基末端卷曲螺旋结构域的相互作用。生化证据证实了Pex19p和Pex17p之间的相互作用。此外，Pex17 p交联到过氧化物酶体靶向信号受体对接复合物的组分上，该复合物意外地含有Pex3p。我们的证据表明，存在不同的亚复合物，包含可分离的池Pex3p，Pex19p，Pex17p，Pex14p，和过氧化物酶体靶向信号受体。这些不同的池可以用于不同的目的，用于输入基质蛋白或PMP。
Pichia pastoris PEX17was cloned by complementation of a peroxisome-deficient strain obtained from a novel screen for mutants disrupted in the localization of a peroxisomal membrane protein (PMP) reporter.PEX17encodes a 267-amino-acid protein with low identity (18%) to the previously characterizedSaccharomyces cerevisiaePex17p. Like ScPex17p, PpPex17p contains a putative transmembrane domain near the amino terminus and two carboxyl-terminal coiled-coil regions. PpPex17p behaves as an integral PMP with a cytosolic carboxyl-terminal domain.pex17Δ mutants accumulate peroxisomal matrix proteins and certain integral PMPs in the cytosol, suggesting a critical role for Pex17p in their localization. Peroxisome remnants were observed in thepex17Δ mutant by morphological and biochemical means, suggesting that Pex17p is not absolutely required for remnant formation. Yeast two-hybrid analysis demonstrated that the carboxyl terminus of Pex19p was required for interaction with Pex17p lacking the carboxyl-terminal coiled-coil domains. Biochemical evidence confirmed the interaction between Pex19p and Pex17p. Additionally, Pex17p cross-linked to components of the peroxisome targeting signal–receptor docking complex, which unexpectedly contained Pex3p. Our evidence suggests the existence of distinct subcomplexes that contain separable pools of Pex3p, Pex19p, Pex17p, Pex14p, and the peroxisome targeting signal receptors. These distinct pools may serve different purposes for the import of matrix proteins or PMPs.
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PEX基因呈上升趋势
影响因子：
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作者：
S. Subramani
通讯作者：
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内质网在过氧化物酶体生物发生中起着重要作用。
DOI：
--
发表时间：
1998
期刊：
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影响因子：
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影响因子：
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作者：
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影响因子：
3.3
作者：
Rieder, SE;Emr, SD
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Emr, SD