Low molecular weight microtubule-associated proteins are light chains of microtubule-associated protein 1 (MAP 1).
Low molecular weight microtubule-associated proteins are light chains of microtubule-associated protein 1 (MAP 1).
复制标题
低分子量微管相关蛋白是微管相关蛋白 1 (MAP 1) 的轻链。
DOI：
10.1073/pnas.80.5.1342
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Davis,SE
中科院分区：
文献类型：
--
作者：
Vallee,RB;Davis,SE
微管相关蛋白1（MAP 1; Mr = 350，000）通过从小牛脑灰质和白色物质获得的微管蛋白的柱层析进行分析。发现两种低分子量蛋白质（LMW MAP; Mr 28，000和30，000）在所有检查条件下与MAP 1共色谱。从小牛脑白色物质中纯化MAP 1和LMW MAP，作为含有约等摩尔量的三种物质的复合物。使用尿素（6 M）从MAP 1中除去LMW MAP。MAP 1与微管的结合不受尿素的影响，并且在有或没有LMW物质的情况下发生。电子显微镜的微管组成的纯化的微管蛋白和MAP 1制剂显示，像MAP 2，MAP 1的微管表面上的丝状臂的外观。
Microtubule-associated protein 1 (MAP 1; Mr = 350,000) was analyzed by column chromatography of microtubule protein obtained from calf brain gray and white matter. Two low molecular weight proteins (LMW MAPs; Mr 28,000 and 30,000) were found to cochromatograph with MAP 1 under all conditions examined. MAP 1 and the LMW MAPs were purified from calf brain white matter as a complex containing approximately equimolar amounts of the three species. Urea (6 M) was used to remove the LMW MAPs from MAP 1. Binding of MAP 1 to microtubules was unaffected by urea and occurred with or without the LMW species. Electron microscopy of microtubules composed of purified tubulin and either MAP 1 preparation revealed that, like MAP 2, MAP 1 has the appearance of a filamentous arm on the microtubule surface.