GANGLIOSIDES OF HUMAN-MELANOMA
GANGLIOSIDES OF HUMAN-MELANOMA
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DOI：
10.1093/jnci/78.1.45
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发表时间：
1987-01-01
影响因子：
10.3
通讯作者：
IRIE, RF
中科院分区：
文献类型：
--
作者：
TSUCHIDA, T;SAXTON, RE;IRIE, RF
本文对80例人恶性黑色素瘤标本（包括52例手术切除标本和28例培养细胞）的神经节苷脂成分进行了研究。从这些组织中分离并纯化神经节苷脂级分，并通过薄层色谱法（TLC）和TLC扫描仪评估各组分神经节苷脂的量。5种神经节苷脂（GM 3、GD 3、GM 2、GD 2和碱不稳定神经节苷脂）在这些黑色素瘤中最常表达。然而，在活组织检查的和培养的黑素瘤中，总神经节苷脂量（范围为33至302 μ g/g组织湿重）以及每种神经节苷脂的分布是广泛不均匀的。当神经节苷脂的表达培养和活检的黑色素瘤进行了比较，GM 2和GD 2的活检的黑色素瘤的次要成分，但往往成为培养的黑色素瘤细胞的主要成分。相反，碱不稳定神经节苷脂表达更强烈的活检黑色素瘤。这种异质性表明，在使用黑色素瘤相关神经节苷脂的单克隆抗体进行黑色素瘤诊断或治疗之前，有必要分析活检黑色素瘤的神经节苷脂组成。
The ganglioside composition of human malignant melanoma was studied with the use of 80 melanoma specimens, including 52 surgical specimens and 28 cultured cell lines. A ganglioside fraction was isolated and purified from each of these tissues, and the amount of each component ganglioside was assessed by thin-layer chromatography (TLC) and a TLC scanner. Five gangliosides (GM3, GD3, GM2, GD2, and alkali-labile ganglioside) were most commonly expressed by these melanomas. However, the total ganglioside amount (ranging from 33 to 302 .mu.g/g wet wt of tissue) as well as the distribution of each ganglioside were widely heterogeneous in both biopsied and cultured melanomas. When the ganglioside expressions of cultured and biopsied melanomas were compared, GM2 and GD2 were minor components of biopsied melanomas but often became major components of cultured melanoma cells. Conversely, alkali-labile ganglioside was expressed more strongly on biopsied melanomas. This heterogeneity suggests that it will be necessary to analyze the ganglioside composition of biopsied melanomas before using monoclonal antibodies to melanoma-associated gangliosides for melanoma diagnosis or therapy.