Simultaneous high-performance liquid chromatography with diode array detection and time-of-flight mass spectrometric confirmation of the ten bioactive compounds in Semen Sojae Preparatum.
Simultaneous high-performance liquid chromatography with diode array detection and time-of-flight mass spectrometric confirmation of the ten bioactive compounds in Semen Sojae Preparatum.
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DOI：
10.1002/jssc.201800504
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发表时间：
2018-08
影响因子：
3.1
通讯作者：
F. Qiu;Linyi Shi;Siqi Wang;Sha Wu;Manyuan Wang
中科院分区：
文献类型：
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作者：
F. Qiu;Linyi Shi;Siqi Wang;Sha Wu;Manyuan Wang
黄豆精是应用最广泛的中药之一。建立了一种可靠、准确的高效液相色谱二极管阵列检测方法，并验证了该方法对制备大豆中10种生物活性成分的定量测定。首先采用80%乙醇在100°C下压力液体萃取15 min，静萃取3次。色谱分离采用C18色谱柱，流动相为水-乙腈，梯度洗脱，检测波长为210 nm。进一步采用高效液相色谱-飞行时间质谱分析系统对样品进行分析，以确认测定结果。10种分析物均分离良好，校准曲线线性良好。日内、日间的相对标准偏差分别为0.20 ~ 1.43%和0.40 ~ 4.78%。加样回收率在96.2 ~ 104.3%之间，相对标准偏差< 3.85%。所建立的高效液相色谱法可作为一种可靠、准确的评价不同产地大豆提取物质量的方法。
Semen Sojae Preparatum is one of the most widely used traditional Chinese medicines. A reliable and accurate high-performance liquid chromatography with diode array detection method has been developed and validated for the quantitative determination of the ten bioactive compounds contained in Semen Sojae Preparatum. The samples were first extracted by pressurized liquid extraction using 80% ethanol at 100°C for 15 min and three static extraction cycles. Chromatographic separation was conducted on a C18 column using a mobile phase consisting of water and acetonitrile under gradient elution, and the detection wavelength was set at 210 nm. The samples were further analyzed on a high-performance liquid chromatography with time-of-flight mass spectrometry system to confirm the determination results. All the ten analytes were well separated, and the calibration curves showed good linearity. The intra- and interday precisions were evaluated in terms of relative standard deviation values within the ranges of 0.20-1.43% and 0.40-4.78%, respectively. The recoveries for the ten analytes were all in the ranges of 96.2-104.3%, with relative standard deviation values < 3.85%. The established high-performance liquid chromatography method could serve as a reliable and accurate method for the quality evaluation of Semen Sojae Preparatum from different origins.