Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy
Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy
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用于自我促进光动力疗法的免疫调节混合生物纳米囊泡
DOI：
10.1007/s12274-021-4050-3
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发表时间：
--
期刊：
影响因子：
9.9
通讯作者：
Hai-Yan Xie
中科院分区：
文献类型：
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作者：
Houli Liu;Yao Lei;weidong Nie;Helin Zhao;Yuzhu Wu;Liping Zuo;Guanghao Wu;Ruili Yang;Hai-Yan Xie
类囊体（Tk）膜在光动力学治疗（PDT）中具有独特的优势，因为它不仅携带丰富的含光敏剂卟啉的叶绿素，而且能产生O2。然而，由于Tk的肿瘤靶向性差和O2产生效率低，目前Tk的治疗性能受到极大限制。在这里，我们报告了一种与M1巨噬细胞衍生的细胞外囊泡（M1 EV）融合的Tk膜免疫调节生物纳米囊泡，用于有效的肿瘤PDT。通过用MlEV挤压菠菜的Tk膜来制备杂化纳米囊泡Tk@Ml。系统研究证实Tk@M1不仅能在肿瘤中积极蓄积，还能有效调节肿瘤失活的免疫微环境。这种活化的“热”肿瘤显著增强了Tk@M1的PDT疗效，这归因于过氧化氢酶催化分解增加的H2O2而增加的O2，为构建有效治疗肿瘤的天然系统提供了新的思路。
Thylakoid (Tk) membranes are of unique superiority in photodynamic therapy (PDT) because they not only carry abundant chlorophylls containing photosensitizer porphyrin but also can produce O2. However, the current therapeutic performance of Tk is dramatically limited because of their poor tumor targeting and inefficient O2production. Here, we report an immunomodulatory bio-nanovesicle of Tk membranes fused with M1 macrophage-derived extracellular vesicles (M1 EV) for efficient PDT of tumors. The hybrid nanovesicle Tk@M1 was prepared by squeezing the Tk membranes of spinach with M1 EV. The systemic study confirmed that Tk@M1 can not only actively accumulate in tumors but also effectively regulate the inactive immune microenvironment of tumors. Such activated “hot” tumors significantly enhance the PDT efficacy of Tk@M1 attributed to the increased O2from catalase catalyzed decomposition of augmented H2O2, providing a novel idea about constructing natural systems for effective tumor treatment.