INTESTINAL ANISAKIASIS - A CASE DIAGNOSED BY MORPHOLOGIC AND IMMUNOLOGICAL METHODS
INTESTINAL ANISAKIASIS - A CASE DIAGNOSED BY MORPHOLOGIC AND IMMUNOLOGICAL METHODS
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DOI：
10.1093/ajcp/90.1.107
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发表时间：
1988-07-01
影响因子：
3.5
通讯作者：
FRIERSON, JG
中科院分区：
文献类型：
--
作者：
SAKANARI, JA;LOINAZ, HM;FRIERSON, JG
美国第四个报告的肠异丝虫病病例的诊断是基于在切除回肠的组织学切片的蠕虫的形态特征。通过放射过敏原吸附试验（RAST）、酶联免疫吸附试验（ELISA）和免疫荧光抗体试验（IFA）检测蠕虫抗体支持诊断。在RAST中，患者血清中的IgE抗体对异尖线虫幼虫抗原有特异性反应，而对蛔虫幼虫抗原无特异性反应。感染后6个月，ELISA检测出异尖线虫幼虫抗原IgG和IgM抗体。IFA结果表明，患者血清能够识别蠕虫产生的排泄-分泌产物，并在体内显示出它们的存在。这些产物似乎来自寄生幼虫的排泄孔和食道背腺。
The diagnosis of the fourth reported case of intestinal anisakiasis in the United States was based on the morphologic characteristics of the worm in histologic sections of resected ileum. Detection of antibodies to the worm by a radioallergo-sorbent test (RAST), enzyme-linked immunosorbent assay (ELISA), and an immunofluorescent antibody assay (IFA) support the diagnosis. IgE antibodies in patient serum reacted specifically to larval Anisakis antigens but not to larval Ascaris antigens in the RAST. IgG and IgM antibodies to larval Anisakis antigen could be detected by ELISA up to six months after infection. IFA findings demonstrated that patient serum recognized the excretory-secretory products produced by the worm and showed their presencein vivo. These products appear to originate from the excretory pore and dorsal esophageal gland of the larval parasite.