Sequencing wheat chromosome arm 7BS delimits the 7BS/4AL translocation and reveals homoeologous gene conservation
Sequencing wheat chromosome arm 7BS delimits the 7BS/4AL translocation and reveals homoeologous gene conservation
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DOI：
10.1007/s00122-011-1717-2
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发表时间：
2012-02-01
影响因子：
5.4
通讯作者：
Edwards, David
中科院分区：
文献类型：
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作者：
Berkman, Paul J.;Skarshewski, Adam;Edwards, David
复杂的小麦族基因组由于其大小和重复性质而对基因组测序工作构成挑战。基因组测序可以揭示相关基因组之间的保守和重排的细节。我们应用Illumina第二代测序技术对普通小麦染色体臂7 BS的低拷贝和独特区域进行测序和组装，然后基于短柄草中的基因顺序构建同线构建。我们已经划定了先前报道的7 BS和4AL之间的易位的位置，分辨率为一个或几个基因，并报告约13%的基因从7 BS已经易位到4AL。在7 BS上发现了另外13个基因，它们似乎起源于4AL。7 DS和7 BS同线构建的基因含量表明小麦中共有77，000个相似基因。在小麦同线区中，7 BS和7 DS共有740个基因，与Brachypodium同源基因的保守率为39%，与Brachypodium共线率为60%。小麦同源基因的比较显示，与先前在7 DS中鉴定的基因相似，84%的基因在7 BS或4AL上具有同源基因。我们已经确定的保守率之间的小麦同源，并与短柄草同源基因保守水平为未来的比较基因组分析提供了一个基准。7 BS的同线性构建可在http://www.wheatgenome.info公开获得，
Complex Triticeae genomes pose a challenge to genome sequencing efforts due to their size and repetitive nature. Genome sequencing can reveal details of conservation and rearrangements between related genomes. We have applied Illumina second generation sequencing technology to sequence and assemble the low copy and unique regions of Triticum aestivum chromosome arm 7BS, followed by the construction of a syntenic build based on gene order in Brachypodium. We have delimited the position of a previously reported translocation between 7BS and 4AL with a resolution of one or a few genes and report approximately 13% genes from 7BS having been translocated to 4AL. An additional 13 genes are found on 7BS which appear to have originated from 4AL. The gene content of the 7DS and 7BS syntenic builds indicate a total of similar to 77,000 genes in wheat. Within wheat syntenic regions, 7BS and 7DS share 740 genes and a common gene conservation rate of similar to 39% of the genes from the corresponding regions in Brachypodium, as well as a common rate of colinearity with Brachypodium of similar to 60%. Comparison of wheat homoeologues revealed similar to 84% of genes previously identified in 7DS have a homoeologue on 7BS or 4AL. The conservation rates we have identified among wheat homoeologues and with Brachypodium provide a benchmark of homoeologous gene conservation levels for future comparative genomic analysis. The syntenic build of 7BS is publicly available at http://www.wheatgenome.info.,