Genetic control of the immune response to nuclease. II. Detection of idiotypic determinants by the inhibition of antibody-mediated nuclease inactivation.
Genetic control of the immune response to nuclease. II. Detection of idiotypic determinants by the inhibition of antibody-mediated nuclease inactivation.
复制标题
对核酸酶免疫反应的遗传控制。
DOI：
10.4049/jimmunol.116.4.959
复制
发表时间：
1976
影响因子：
4.4
通讯作者：
D. H. Sachs
中科院分区：
文献类型：
--
作者：
C. Fathman;D. H. Sachs
小鼠对葡萄球菌核酸酶的体液反应以前被证明是由H-2连锁的ir基因控制的(S)。为了检测可变区免疫球蛋白基因对这种遗传控制的可能贡献，我们开发了一个检测抗核酸酶免疫球蛋白分子独特型决定簇的系统。A/J和SJL两株高应答株均能产生核酸酶抗血清。用琼脂糖核酸酶柱亲和层析法纯化相应抗体，免疫Lewis大鼠。建立了一种检测系统，以评估由此产生的大鼠抗血清对抗体介导的核酸酶活性失活的抑制作用。尽管在这些血清中存在许多物种特异性的抗鼠免疫球蛋白抗体，但对抗体介导的酶失活的抑制被发现是免疫菌株的抗核酸酶抗体所特有的。这种抑制不能通过广泛吸收产生抗体的菌株的正常血清蛋白来消除，并且被证明需要针对抗核酸酶抗体的结合部位的抗体。因此，这种抑制作用定义了抗核酸酶抗体的独特型决定因素。
The humoral response of mice to staphylococcal nuclease has previously been shown to be controlled genetically by H-2-linked Ir gene(s). In order to examine the possible contributions of variable region immunoglobulin genes to this genetic control, we have developed a system for the detection of idiotypic determinants on anti-nuclease immunoglobulin molecules. Antisera to nuclease were raised in two high responder strains, A/J and SJL. The corresponding antibodies were purified by affinity chromotography on Sepharose-nuclease columns, and were used to immunize groups of Lewis rats. An assay system was developed to assess the inhibition of antibody-mediated inactivation of nuclease activity by the rat antisera thus produced. Despite the presence of many species-specific anti-mouse immunoglobulin antibodies in these sera, inhibition of antibody-mediated enzyme inactivation was found to be specific for anti-nuclease antibodies of the immunizing strain. The inhibition could not be removed by extensive absorption with normal serum proteins from the antibody-producing strain, and was shown to require antibodies directed toward binding sites of the anti-nuclease antibodies. This inhibition thus defines idiotypic determinants of anti-nuclease antibodies.