Application of small angle X-ray scattering in exploring the effect of edible oils with different unsaturation FAs on bioaccessibility of stigmasterol oleate
Application of small angle X-ray scattering in exploring the effect of edible oils with different unsaturation FAs on bioaccessibility of stigmasterol oleate
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DOI：
10.1002/jsfa.12874
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发表时间：
2023-08-11
影响因子：
4.1
通讯作者：
Chang，Ming
中科院分区：
文献类型：
--
作者：
Wang，Ying;Wang，Tao;Chang，Ming
背景植物甾醇通过与脂肪酸的酯化反应改善其脂类溶解性、亲油性/亲水性平衡和生物可获得性，扩大了其在食品工业中的实际应用范围。本文采用小角X射线散射法，结合pH-稳态体外消化模型，连续监测混合胶束在消化过程中的分子结构演变，研究了3种食用油(油酸含量为72.41±0.57%的橄榄油、亚油酸含量为63.45±0.78%的葵花籽油、亚麻酸含量为51.74±0.34%的精制亚麻油)对豆油醇油酸酯体外生物可达性的影响。在模拟豆油醇油酸酯体外消化过程中，发现三种不同的食用油对饲料混合胶束的形成有相似的影响，但混合胶束的分子形态和组成存在显著差异。结果表明，亚油酸(SO体系)或亚麻酸(LO体系)形成的囊泡容易解离。与亚油酸(SO系统)和亚麻酸(LO系统)相比，油酸(OO系统)可以促进胶束向囊泡的转化，并保持其膜的稳定性，从而显著促进豆甾醇的溶解，提高生物可利用性。这篇文章受版权保护。版权所有。
BackgroundThe phytosterol can improve its lipid solubility, lipophilic/hydrophilic balance and bioaccessibility by esterification with fatty acids, which increases its practical application range in food industry. In this article, small angle X-ray scattering (SAXS) combined with pH-stat vitro digestion model is applied to continuously monitor the molecular structure evolution of mixed micelles during digestion and study the effect of three edible oils (olive oil with 72.41±0.57% oleic, sunflower seed oil with 63.45±0.78% linoleic, refined linseed oil with 51.74±0.34% linolenic) on bioaccessibility of stigmasterol oleate in vitro.ResultsCompare the release degree and rate of fatty acids in the three edible oil systems (k OO+ ST-OA= 0.0501, k SO+ ST-OA= 0.0357, k LO+ ST-OA= 0.0323). It is found that the three different edible oils have similar impact on formation of dietary mixed micelles during the simulated vitro-digestion of stigmasterol oleate, but there are significant differences in molecular morphology and composition of mixed micelles. The results show that the vesicles formed by linoleic (SO system) or linolenic (LO system) were easy to dissociate. While the largest average number and diameter of vesicles, 5.55× 1016 cm-3 and 2230.75 Å, the most stable vesicle structure and the fastest fatty acid release rate are observed in OO system.ConclusionCompared to linoleic (SO system) or linolenic (LO system), the oleic (OO system) could facilitate the transformation of micelles to vesicles and maintain the stability of its membrane, then to significantly promote dissolution of stigmasterol and improve bioaccessibility. This article is protected by copyright. All rights reserved.