Does Enhancement of Oxidative Stress Markers Mediate Health Effects of Ambient Air Particles?
Does Enhancement of Oxidative Stress Markers Mediate Health Effects of Ambient Air Particles?
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DOI：
10.1089/ars.2013.5694
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发表时间：
2014-07-01
影响因子：
6.6
通讯作者：
Violi, Francesco
中科院分区：
文献类型：
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作者：
Bertazzi, Pier Alberto;Cantone, Laura;Violi, Francesco
有证据表明，氧化应激的产生可能导致与颗粒物（PM）接触相关的健康影响。我们调查了113名暴露于富含金属的PM的工人和61名未暴露的志愿者的氧化应激标志物。分析血浆可溶性nox2衍生肽（sNOX2-dp）和两种氧化应激标志物尿8-羟基脱氧鸟苷（8-OHdG）和8-异-前列腺素2 α (8-iso-PGF2 α)的水平。还评估了抗氧化剂α -生育酚的血浆水平。工人在工作场所接触空气动力学直径< 10 μ m （PM10）的平均暴露量远高于志愿者居住的城市。工人尿中8-iso-PGF2 α、8-OHdG和血浆sNOX2-dp水平明显高于未暴露者。工人体内的α -生育酚含量远低于未暴露的人。在调整了年龄、体重指数和吸烟因素的多变量回归模型中，工人体内8-iso-PGF2 α的升高与PM10和金属暴露有关；8-OHdG和sNOX2-dp仅与铁有关。α -生育酚与各氧化应激标志物呈负相关。我们的观察得出这样的假设，即与暴露于高富金属PM水平相关的氧化应激标志物的增强代表了从颗粒暴露到系统（例如心血管）影响的途径中的一个可能步骤。
Evidence indicates that oxidative stress generation may contribute to health effects associated with particulate matter (PM) exposure. We investigated oxidative stress markers in 113 workers exposed to metal-rich PM and 61 nonexposed comparable volunteers. The plasma levels of soluble NOX2-derived peptide (sNOX2-dp) and two oxidative stress markers, urinary 8-hydroxydeoxyguanosine (8-OHdG) and 8-iso-prostaglandinF2alpha (8-iso-PGF2 alpha), were analyzed. The plasma levels of the antioxidant alpha-tocopherol were also evaluated. The workers' average exposure to PM with an aerodynamic diameter < 10 mu m (PM10) was much higher at the workplace than in the city, where the volunteers lived. Workers had significantly higher urinary 8-iso-PGF2 alpha and 8-OHdG and plasma sNOX2-dp levels than nonexposed subjects. Alpha-tocopherol was much lower in workers compared with nonexposed subjects. In multivariable regression models adjusted for age, body mass index, and smoking, 8-iso-PGF2 alpha increased in workers in association with PM10 and metal exposure; 8-OHdG and sNOX2-dp were associated only with iron. Alpha-tocopherol was inversely associated with each of the oxidative stress markers. Our observation leads to the hypothesis that the enhancement of oxidative stress markers associated with exposure to high metal-rich PM levels represents a possible step in the pathways leading from particle exposure to systemic (e.g., cardiovascular) effects.