MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3
MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3
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DOI：
10.4238/2013.april.2.17
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发表时间：
2013-01-01
影响因子：
0.4
通讯作者：
Hao, C. Q.
中科院分区：
文献类型：
--
作者：
He, L.;Yao, H.;Hao, C. Q.
我们检测了microRNA-181b(MiRNA)在前列腺癌组织中的表达及其对前列腺癌PC-3细胞的影响。手术切除前列腺癌组织27例，正常前列腺组织30例。提取总miRNA，用RT-PCR方法检测miR-181b的相对表达。将miR-181b反义寡核苷酸基因导入前列腺癌PC-3细胞。用RT-PCR法检测MIR-181b在转染组和未转染组细胞中的表达。用流式细胞仪检测细胞凋亡率的变化。采用四甲基偶氮唑盐比色法和细胞生长曲线法检测miR-181b表达对细胞增殖的影响。用Transwell小室方法检测细胞体外侵袭能力的变化。与正常前列腺组织相比，MIR-181b在前列腺癌组织中表达上调。将miR-181b反义寡核苷酸导入前列腺癌PC-3细胞后，其表达下调。下调miR-181b可诱导PC-3细胞凋亡，抑制细胞增殖，抑制PC-3细胞体外侵袭。由于miR-181b在前列腺癌中过表达，其下调可能通过诱导细胞凋亡、抑制增殖和抑制癌细胞的侵袭而成为前列腺癌的基因治疗手段。
We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC-3 cells. miR-181b expression in transfected and non-transfected cells was measured using RT-PCR. Changes in cell apoptosis were measured using flow cytometry. MTT and cell growth curve methods were used to assess the influence of miR-181b expression on cell proliferation. The changes in cell invasive ability in vitro were detected using the Transwell chamber method. miR-181b was up-regulated in the prostate cancer tissues compared with the normal prostate samples. It was down-regulated after miR-181b ASO transfection into the prostate cancer PC-3 cells. Down-regulation of miR-181b in the PC-3 cell induced apoptosis, inhibited proliferation, and depressed invasion of PC-3 cells in vitro. As miR-181b is over-expressed in prostate cancer, its down-regulation could have potential as gene therapy for prostate cancer by inducing apoptosis, inhibiting proliferation and depressing invasion by cancer cells.