Carbonate Apatite Micro-Honeycombed Blocks Generate Bone Marrow-Like Tissues as well as Bone
Carbonate Apatite Micro-Honeycombed Blocks Generate Bone Marrow-Like Tissues as well as Bone
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DOI：
10.1002/adbi.201900140
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发表时间：
2019-09-30
影响因子：
4.1
通讯作者：
Ishikawa, Kunio
中科院分区：
文献类型：
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作者：
Hayashi, Koichiro;Kishida, Ryo;Ishikawa, Kunio
造血干细胞在骨髓中形成血细胞并驻留在小生境中。保存这些小生境的人工环境可能会产生骨髓。骨生成、血管生成和材料再吸收必须被调节以创造这些环境。这些过程由材料组成和宏观及微孔结构控制。在这里，三个块具有不同的MEMS结构制造。碳酸磷灰石具有与天然人骨几乎相同的组成，并且它们的蜂窝结构有利于细胞渗透和存活。在具有高微孔性的样品中，在材料吸收和高局部钙浓度的区域形成骨内膜样组织，例如窦状隙。这些条件类似于有利于生态位维持的环境。骨髓样组织和巨核细胞在这种环境中成功地产生。微孔结构是骨髓形成的最关键因素，但也必须考虑材料组成和大孔结构的影响。这项研究的结果可能有助于开发骨髓相关疾病的治疗方法，并阐明造血干细胞生态位的组成和功能。
Hematopoietic stem cells form blood cells in bone marrow and reside in niches. Artificial environments that conserve these niches may generate bone marrow. Osteogenesis, angiogenesis, and material resorption must be regulated to create these environments. These processes are controlled by material composition and macro- and microporous structures. Here, three blocks with different micropore structures are fabricated. Carbonate apatite has nearly the same composition as natural human bone and their honeycomb structure facilitates cell penetration and survival. In samples with high microporosity, endosteum-like tissues such as sinusoids form in areas of material resorption and high local calcium concentration. These conditions resemble environments conducive to niche maintenance. Bone marrow-like tissues and megakaryocytes are successfully generated in this environment. Micropore structure is the most critical factor in bone marrow formation; however, the influences of material composition and macropore structure must also be considered. The results of this study may help develop treatments for bone marrow-related diseases and elucidate the components and functions of the hematopoietic stem cell niche.