Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones.
Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones.
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橡胶乳胶的比较蛋白质组学揭示了不同橡胶树克隆中 REF/SRPP 家族的多种蛋白质对乙烯刺激的不同反应
DOI：
10.3390/ijms18050958
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发表时间：
2017-05-02
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Tong Z;Wang D;Sun Y;Yang Q;Meng X;Wang L;Feng W;Li L;Wurtele ES;Wang X
橡胶伸长因子（REF）和橡胶小颗粒蛋白（SRPP）是天然橡胶生物合成的两个关键因子。为了进一步了解这些蛋白在橡胶形成中的作用，从橡胶树Reyan（RY）7-33-97中鉴定了胶乳丰富REF或SRPP蛋白的六个不同基因，包括REF 138、175、258和SRPP 117、204、243。序列分析表明，REF具有可变的和长的N-末端，而SRPP在REF结构域之外具有可变的和长的C-末端，并且REF 258在其N-末端具有ATP酶的β亚基。双向电泳（2-DE）结果显示，REF/SRPP蛋白在2-DE凝胶上分离为多个蛋白质点，表明它们具有多个蛋白质种类。REF/SRPP蛋白质的丰度进行了比较乙烯和对照处理之间或橡胶树无性系与不同水平的胶乳生产力通过分析2-DE凝胶。在乙烯刺激下，每个REF/SRPP蛋白的总丰度降低或变化不大，而同一REF/SRPP的多个蛋白种类的丰度变化很大。3个橡胶树无性系间蛋白质种类的丰度也存在显著差异。特别地，REF 175或REF 258的两种蛋白质种类仅在高胶乳生产力克隆RY 8-79中而不是在RY 7-33-97和PR 107中是乙烯响应的。有些蛋白质种类与乙烯刺激和胶乳产量呈正相关。这些结果表明，橡胶树中特定的蛋白质种类可能比其他蛋白质种类更重要，翻译后修饰可能在橡胶树的生物合成中起重要作用。
Rubber elongation factor (REF) and small rubber particle protein (SRPP) are two key factors for natural rubber biosynthesis. To further understand the roles of these proteins in rubber formation, six different genes for latex abundant REF or SRPP proteins, including REF138,175,258 and SRPP117,204,243, were characterized from Hevea brasiliensis Reyan (RY) 7-33-97. Sequence analysis showed that REFs have a variable and long N-terminal, whereas SRPPs have a variable and long C-terminal beyond the REF domain, and REF258 has a β subunit of ATPase in its N-terminal. Through two-dimensional electrophoresis (2-DE), each REF/SRPP protein was separated into multiple protein spots on 2-DE gels, indicating they have multiple protein species. The abundance of REF/SRPP proteins was compared between ethylene and control treatments or among rubber tree clones with different levels of latex productivity by analyzing 2-DE gels. The total abundance of each REF/SRPP protein decreased or changed a little upon ethylene stimulation, whereas the abundance of multiple protein species of the same REF/SRPP changed diversely. Among the three rubber tree clones, the abundance of the protein species also differed significantly. Especially, two protein species of REF175 or REF258 were ethylene-responsive only in the high latex productivity clone RY 8-79 instead of in RY 7-33-97 and PR 107. Some individual protein species were positively related to ethylene stimulation and latex productivity. These results suggested that the specific protein species could be more important than others for rubber production and post-translational modifications might play important roles in rubber biosynthesis.