A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging.
A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging.
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DOI：
10.1038/ncomms15269
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发表时间：
2017-05-19
影响因子：
16.6
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Antaris AL;Chen H;Diao S;Ma Z;Zhang Z;Zhu S;Wang J;Lozano AX;Fan Q;Chew L;Zhu M;Cheng K;Hong X;Dai H;Cheng Z
第二近红外窗口（NIR-II）中的荧光成像允许以前所未有的清晰度可视化深层解剖特征。NIR-II荧光团来自广泛的材料，涵盖半导体纳米材料到有机分子染料，但不幸的是，所有发射> 1，000 nm的水溶性有机分子都具有低量子产率，其具有有限的时间分辨率和穿透深度。在这里，我们报告定制的超分子组装蛋白质复合物与磺化NIR-II有机染料（CH-4 T）产生辉煌的110倍的荧光增加，从而导致迄今为止最高的量子产率分子荧光团。明亮的分子复合物允许在第二个NIR窗口中进行最快的视频速率成像，并且能够以快速50帧每秒（FPS）的速度将曝光时间减少50倍，从而能够解析小鼠心动周期。此外，我们证明了NIR-II分子复合物上级临床批准的ICG用于小鼠体内深处的淋巴结成像。> 1，000 nm的近红外（NIR）荧光成像允许深层组织成像，但可用的有机染料显示出较差的亮度和时间分辨率。在这里，作者合成了一种近红外染料，在结合血清蛋白时，其强度增加了110倍，量子产率为11%。
Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with >1,000 nm emission suffer from low quantum yields that have limited temporal resolution and penetration depth. Here, we report tailoring the supramolecular assemblies of protein complexes with a sulfonated NIR-II organic dye (CH-4T) to produce a brilliant 110-fold increase in fluorescence, resulting in the highest quantum yield molecular fluorophore thus far. The bright molecular complex allowed for the fastest video-rate imaging in the second NIR window with ∼50-fold reduced exposure times at a fast 50 frames-per-second (FPS) capable of resolving mouse cardiac cycles. In addition, we demonstrate that the NIR-II molecular complexes are superior to clinically approved ICG for lymph node imaging deep within the mouse body. Near-infrared (NIR) fluorescence imaging >1,000 nm allows deep tissue imaging, but available organic dyes display poor brightness and temporal resolution. Here, the authors synthesize a NIR dye that, upon binding serum proteins, exhibits a 110-fold increase in intensity, giving an 11% quantum yield.