Further isolation and characterization of temperature-sensitive mutants of influenza virus.
Further isolation and characterization of temperature-sensitive mutants of influenza virus.
复制标题
流感病毒温度敏感突变体的进一步分离和表征。
DOI：
10.1016/0042-6822(75)90042-2
复制
发表时间：
1975
期刊：
影响因子：
3.7
通讯作者：
C. Enomoto
中科院分区：
文献类型：
--
作者：
A. Sugiura;M. Ueda;K. Tobita;C. Enomoto
通过两两杂交，将流感病毒WSN株的34个温度敏感（ts）突变体划分为7个不重叠的重组互补组。在27个突变体中，病毒基因组的单个基因受到影响，6个是双突变体，1个是三重突变体。通过在放线菌素D存在下将[3H]尿苷掺入酸不溶性产物中，研究了在非允许温度下突变感染细胞中病毒粒子RNA的合成。这种方法将突变体分为两类。属于I、II、III和V组的突变体为RNA−，属于IV、VI和VII组的突变体为RNA+。温度升高实验结果表明，所有四组RNA -突变体在复制病毒粒子RNA方面都存在缺陷。RNA -突变体在不允许温度下产生的血清学或功能上活跃的亚病毒成分（核糖核蛋白、血凝素和神经氨酸酶）是可变的。RNA+突变体产生正常数量的这些成分，但IV组突变体的功能性血凝素和神经氨酸酶的产生被大大抑制。
We have assigned 34 temperature-sensitive (ts) mutants of the WSN strain of influenza virus into seven nonoverlapping recombination-complementation groups by conducting pairwise crosses. A single gene of the viral genome was affected in 27 mutants while six were double mutants and one was a triple mutant. The synthesis of virion RNA in mutant-infected cells at the nonpermissive temperature was studied by the incorporation of [3H]uridine in the presence of actinomycin D into an acid-insoluble product. This method separated mutants into two classes. Mutants belonging to Groups I, II, III, and V were RNA−, while those belonging to Groups IV, VI, and VII were RNA+. The results of temperature shift-up experiments suggested that all four groups of RNA−mutants were defective in replicating virion RNA. Production of serologically or functionally active subviral components (ribonucleoprotein, hemagglutinin, and neuraminidase) by RNA−mutants at the nonpermissive temperature was variable. RNA+mutants produced a normal amount of these components, except Group IV mutants in which the production of functional hemagglutinin and neuraminidase was greatly depressed.