BLOCK OF CA2+ WAVE AND CA2+ OSCILLATION BY ANTIBODY TO THE INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR IN FERTILIZED HAMSTER EGGS
BLOCK OF CA2+ WAVE AND CA2+ OSCILLATION BY ANTIBODY TO THE INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR IN FERTILIZED HAMSTER EGGS
复制标题
DOI：
10.1126/science.1321497
复制
发表时间：
1992-07-10
期刊：
影响因子：
56.9
通讯作者：
MIKOSHIBA, K
中科院分区：
文献类型：
--
作者：
MIYAZAKI, S;YUZAKI, M;MIKOSHIBA, K
在各种受刺激的细胞中，胞浆内游离钙(Ca~(2+))浓度以波(Ca~(2+)波)和周期性瞬变(Ca~(2+)振荡)的形式增加。这些现象可以用肌醇1，4，5-三磷酸(IP3)诱导的细胞内钙释放(IICR)和钙离子诱导的细胞内钙释放(CICR)来解释，但缺乏决定性的证据。抗IP3受体的单抗分别抑制IP3和Ca~(2+)注入金黄地鼠卵的IICR和CICR。该抗体完全阻断了精子诱导的钙波和钙振荡。结果表明，地鼠受精卵中钙的释放仅由IP3受体介导，钙敏化的IICR而不是CICR产生钙波和钙振荡。
The concentration of cytoplasmic free calcium (Ca2+) increases in various stimulated cells in a wave (Ca2+ wave) and in periodic transients (Ca2+ oscillations). These phenomena are explained by inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) and Ca2+-induced Ca2+ release (CICR) from separate intracellular stores, but decisive evidence is lacking. A monoclonal antibody to the IP3 receptor inhibited both IICR and CICR upon injection of IP3 and Ca2+ into hamster eggs, respectively. The antibody completely blocked sperm-induced Ca2+ waves and Ca2+ oscillations. The results indicate that Ca2 release in fertilized hamster eggs is mediated solely by the IP3 receptor, and Ca2+-sensitized IICR, but not CICR, generates Ca2+ waves and Ca2+ oscillations.