Regulation of cell-surface genes and biofilm formation by the C-albicans transcription factor Bcr1p
Regulation of cell-surface genes and biofilm formation by the C-albicans transcription factor Bcr1p
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DOI：
10.1016/j.cub.2005.05.047
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发表时间：
2005-06-21
期刊：
影响因子：
9.2
通讯作者：
Mitchell, AP
中科院分区：
文献类型：
--
作者：
Nobile, CJ;Mitchell, AP
许多微生物对其环境的影响取决于它们形成称为生物膜的表面结合群落的能力[1]。植入式医疗器械上的生物膜形成为侵入性细菌和真菌病原体提供了入口和避难所，对人类健康造成了严重后果[1，2]。生物膜调节剂和粘附分子被广泛定义用于许多细菌病原体[3-5]，但不用于真菌病原体，如白色念珠菌。被称为菌丝的细长丝状体是C.白念珠菌生物膜和促进生物膜形成的已知基因是菌丝发育所需的[2，6-8]。从一个新的转录因子突变体库中，我们确定了Bcr 1 p，一种形成生物膜而不是菌丝所需的锌指蛋白。表达分析表明，Bcr 1 p激活细胞表面蛋白和粘附素基因，包括在菌丝发育过程中诱导的几个。BCR 1的表达依赖于菌丝调节因子Tec 1 p。因此，BCR 1是菌丝调控网络的下游组分，其将细胞表面基因的表达与菌丝分化偶联。我们的结果表明菌丝细胞专门用于呈现支持生物膜完整性的粘附分子。
The impact of many microorganisms on their environment depends upon their ability to form surface bound communities called biofilms [1]. Biofilm formation on implanted medical devices has severe consequences for human health by providing both a portal of entry and a sanctuary for invasive bacterial and fungal pathogens [1, 2]. Biofilm regulators and adherence molecules are extensively defined for many bacterial pathogens [3-5], but not for fungal pathogens such as Candida albicans. Elongated filaments called hyphae are a prominent feature of C. albicans biofilms, and known genes that promote biofilm formation are required for hyphal development [2, 6-8]. From a new library of transcription-factor mutants' we identify Bcr1p, a zinc finger protein required for formation of biofilms but not hyphae. Expression analysis shows that Bcr1p activates cell-surface protein and adhesin genes, including several induced during hyphal development. BCR1 expression depends upon the hyphal regulator Tec1p. Thus, BCR1 is a downstream component of the hyphal regulatory network that couples expression of cell-surface genes to hyphal differentiation. Our results indicate that hyphal cells are specialized to present adherence molecules that support biofilm integrity.