Preparation of simian foamy virus type-1 vectors.
Preparation of simian foamy virus type-1 vectors.
复制标题
猿猴泡沫病毒1型载体的制备。
DOI：
10.1101/pdb.prot065516
复制
发表时间：
2011
影响因子：
--
通讯作者：
Mergia,Ayalew
中科院分区：
文献类型：
--
作者：
Park,Jeonghae;Mergia,Ayalew
DISCUSSIONFVs are a group of retroviruses that belong to the Spumaretrovirinae subfamily. These viruses are found in many mammalian species and are nonpathogenic in naturally infected and experimentally infected animals. FVs can be propagated efficiently in various cell types of several species (Mergia et al. 1996; Hill et al. 1999). Humans do not harbor FVs except for accidental occupational infections of animal handlers (Schweizer et al. 1995, 1997; Heneine et al. 1998; Callahan et al. 1999; Wolfe et al. 2004). We have molecularly characterized SFV-1 originally isolated from a macaque monkey (Fig. 1). SFV-1 has a complex genome that encodes the virion structural genes, gag, pol, and env, as well as two large open reading frames (ORFs) located at the 3′ end of env (Mergia et al. 1991; Mergia and Wu 1998). Other strains of FVs also have two ORFs in the same region (Flugel et al. 1987; Maurer and Flugel 1988; Renne et al. 1993; Herchenroder et al. 1994; Renshaw and Casey 1994; Winkler et al. 1997; Tobaly-Tapiero et al. 2000). Viral gene expression in FV is temporally regulated and involves differential gene expression controlled by two promoters. An internal promoter at the 3′ end of env regulates the expression of the early gene products Tas and Bet (Lochelt et al. 1993, 1994; Campbell et al. 1994; Mergia 1994). Tas is encoded by the first ORF located between env and the long terminal repeat (LTR), overlapping the 3′ end of env. It is a transcriptional trans-activator that strongly augments gene expression directed by viral promoters (Keller et al. 1991; Mergia et al. 1991; Rethwilm et al. 1991; Venkatesh et al. 1991; Renne et al. 1993; Renshaw and Casey 1994; Herchenroder et al. 1995). Bet is a product of a spliced message containing the first 88 amino acids of Tas fused to the last 390 amino acids of Orf-2 (Muranyi and Flugel 1991; Hahn et al. 1994; Mergia 1994). Mutational analysis in FVs has revealed that the tas gene is essential for viral replication (Lochelt et al. 1991; Baunach et al. 1993; Mergia and Wu 1998), whereas the orf-2 region is dispensable (Lochelt et al. 1991; Baunach et al. 1993; Mergia and Wu 1998). Different functions have been reported for Bet (Meiering and Linial 2002; Lochelt 2003), suggesting multiple roles of this protein in FV replication. However, because the orf-2 region is not critical, Bet is also dispensable for virus replication in a cell culture system.
