Total-Body Irradiation of Postpubertal Mice with 137Cs Acutely Compromises the Microarchitecture of Cancellous Bone and Increases Osteoclasts
Total-Body Irradiation of Postpubertal Mice with 137Cs Acutely Compromises the Microarchitecture of Cancellous Bone and Increases Osteoclasts
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DOI：
10.1667/rr1463.1
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发表时间：
2009-03-01
影响因子：
3.4
通讯作者：
Globus, Ruth K.
中科院分区：
文献类型：
--
作者：
Kondo, Hisataka;Searby, Nancy D.;Globus, Ruth K.
电离辐射可导致大量组织变性，这可能威胁宇航员和放疗患者的长期健康。为了确定单次剂量的辐射是否会严重损害青春期后骨骼的结构完整性，将18周龄的雄性小鼠暴露于Cs-137 γ辐射（1或2戈伊）。在照射后3或10天以及基础对照组（照射时采集的组织）和年龄匹配的对照组中，通过显微计算机断层扫描（microCT）分析高转换松质骨的结构。照射（2戈伊）导致胫骨松质骨体积分数（BV/TV）在3天内下降20%，在10天内下降43%，而1戈伊导致10天后下降28%。BV/TV减小是由于小梁间距增加和厚度减小。辐射还增加了（类似于150%）内衬抗酒石酸盐、酸性磷酸酶阳性破骨细胞的松质表面，这是骨吸收增加的一个指标。照射后1个月，放射降低腰椎BV/TV，表明松质骨丢失持续存在，尽管压缩力学性能不受影响。总之，单次剂量的γ射线快速增加松质骨组织中的破骨细胞表面，并损害青春期后小鼠重建骨外和中轴骨骼中的松质骨微结构。(C)2009年，辐射研究学会
Ionizing radiation can cause substantial tissue degeneration, which may threaten the long-term health of astronauts and radiotherapy patients. To determine whether a single dose of radiation acutely compromises structural integrity in the postpubertal skeleton, 18-week-old male mice were exposed to Cs-137 gamma radiation (1 or 2 Gy). The structure of high-turnover, cancellous bone was analyzed by microcomputed tomography (microCT) 3 or 10 days after irradiation and in basal controls (tissues harvested at the time of irradiation) and age-matched controls. Irradiation (2 Gy) caused a 20% decline in tibial cancellous bone volume fraction (BV/TV) within 3 days and a 43% decline within 10 days, while 1 Gy caused a 28% reduction 10 days later. The BV/TV decrement was due to increased spacing and decreased thickness of trabeculae. Radiation also increased (similar to 150%) cancellous surfaces lined with tartrate-resistant, acid phosphatase-positive osteoclasts, an index of increased bone resorption. Radiation decreased lumbar vertebral BV/TV 1 month after irradiation, showing the persistence of cancellous bone loss, although mechanical properties in compression were unaffected. In sum, a single dose of gamma radiation rapidly increased osteoclast surface in cancellous tissue and compromised cancellous microarchitecture in the remodeling appendicular and axial skeleton of postpubertal mice. (C) 2009 by Radiation Research Society