Comparison of C 60 and GCIB primary ion beams for the analysis of cancer cells and tumour sections
Comparison of C 60 and GCIB primary ion beams for the analysis of cancer cells and tumour sections
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C 60 和 GCIB 一次离子束在癌细胞和肿瘤切片分析中的比较
DOI：
10.1002/sia.4874
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发表时间：
2012
影响因子：
1.7
通讯作者：
Fletcher J
中科院分区：
文献类型：
--
作者：
Fletcher J
我们已经实施了由Ionoptika有限公司（南安普顿，英国）开发的气体团簇离子束（GCIB）系统，该系统具有足够的控制能力，使我们能够利用我们的J105仪器的独特功能进行成像和深度分析。J105允许我们使用GCIB作为连续的初级离子束，从而克服了与脉冲这些缓慢移动的混合粒子束相关的问题。我们在同一仪器上对相同样品进行了与C60离子的直接比较。GCIB束比C60+离子束更难聚焦，使得单细胞成像困难，尽管Ar 1000和Ar 2000很容易获得15-20 µm的光斑尺寸，为组织切片/活检样本的更大面积成像提供了良好的分辨率。在本文中，我们提出的结果，从评估这些新的光束作为初级离子的分析“真正的”，复杂的生物系统。比较了沉积在硅上的体外培养细胞和体内来源的冷冻切片肿瘤样品的初始光谱和增加初级离子轰击后的光谱。与C60+分析相比，Ar 1000+和Ar 2000+显示来自磷脂的完整分子离子的信号的持续性增加，并且化学背景噪声的积累减少。版权所有© 2012约翰威利父子有限公司.
We have implemented a gas cluster ion beam (GCIB) system developed by Ionoptika Ltd (Southampton, UK) with sufficient control to allow us to exploit the unique capabilities of our J105 instrument for imaging and depth profiling. The J105 allows us to use the GCIB as continuous primary ion beam, thereby overcoming the issues associated with pulsing these slow moving, mixed species beams. We have performed a direct comparison with C60ions on the same samples in the same instrument. The GCIB beams are more difficult to focus than the C60+ion beam, making single‐cell imaging difficult, although spot sizes of 15–20 µm are readily obtainable for Ar1000and Ar2000, providing good resolution for larger area imaging on tissue section/biopsy samples. In this paper, we present results from the assessment of these new beams as primary ions for the analysis of ‘real’, complex biological systems. Initial spectra and those following increased primary ion bombardment were compared forin vitrocultured cells deposited on silicon and cryo‐sectioned tumour samples originatingin vivo. Ar1000+and Ar2000+showed increased persistence of the signals from intact molecular ions of phospholipids and a reduction in the accumulation of chemical background noise compared with C60+analysis. Copyright © 2012 John Wiley & Sons, Ltd.