Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey
Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey
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DOI：
10.1021/acsnano.1c01600
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发表时间：
2021-06-21
期刊：
影响因子：
17.1
通讯作者：
Wegner, Seraphine, V
中科院分区：
文献类型：
--
作者：
Chakraborty, Taniya;Wegner, Seraphine, V
细胞通常通过可扩散的分子相互交流，但非化学交流仍然难以捉摸。虽然生物发光生物通过光来寻找猎物或吸引配偶，但通过光进行信号传递是否可能在细胞水平上仍存在争议。在这里，我们证明了细胞到细胞的信号通过光是可能的，在人工细胞社区来自仿生囊泡。在我们的设计中，人工发送细胞产生细胞内的光信号，触发对接收细胞的粘附。与可溶性分子不同，光信号传播迅速，不依赖于扩散，也不需要跨膜转运蛋白。为了获得一个捕食者-猎物的关系，发光的捕食者细胞装载有一个次要的可扩散的毒药，这是转移到猎物细胞粘附后，导致其裂解。该设计为基于光的细胞间通信提供了蓝图，其可用于对人工和自然细胞群落进行编程。
Cells commonly communicate with each other through diffusible molecules but nonchemical communication remains elusive. While bioluminescent organism , communicate through light to find prey or attract mates, it is still under debate if signaling through light is possible at the cellular level. Here, we demonstrate that cell to cell signaling through light is possible in artificial cell communities derived from biomimetic vesicles. In our design, artificial sender cells produce an intracellular light signal, which triggers the adhesion to receiver cells. Unlike soluble molecules, the light signal propagates fast, independent of diffusion and without the need for a transporter across membranes. To obtain a predator-prey relationship, the luminescence predator cells is loaded with a secondary diffusible poison, which is transferred to the prey cell upon adhesion and leads to its lysis. This design provides a blueprint for light based intercellular communication, which can be used for programing artificial and natural cell communities.