Distinction between antigen receptor and IL 2 receptor triggering events in the activation of alloreactive T cell clones with calcium ionophore and phorbol ester.
Distinction between antigen receptor and IL 2 receptor triggering events in the activation of alloreactive T cell clones with calcium ionophore and phorbol ester.
复制标题
使用钙离子载体和佛波酯激活同种反应性 T 细胞克隆时抗原受体和 IL 2 受体触发事件的区别。
作者：
F. Albert;C. Hua;A. Truneh;M. Pierres;A. Schmitt
先前的研究表明，ca++离子载体与12- o -十四烷酰基磷酸酯（TPA）联合可诱导正常T淋巴细胞表达T细胞生长因子白细胞介素2 （IL - 2）受体，分泌IL - 2并增殖(1)。在这里，我们使用长期同种异体反应Lyt-2+细胞毒性或T4+“辅助”T细胞克隆。在没有外源性IL - 2的情况下，所有的克隆都分泌ifn - γ，但只有T4+克隆分泌IL - 2并对适当的同种异体抗原做出反应。Ca++离子载体离子霉素和TPA联合使用，模拟了特异性异体抗原对这些T细胞克隆的作用，即它们诱导所有克隆分泌ifn - γ，诱导T4+克隆分泌IL - 2。在缺乏外源IL - 2的情况下，仅对IL - 2分泌克隆诱导增殖反应。在同种异体抗原或碘霉素和TPA处理后，一些T细胞克隆对外源性IL - 2的敏感性也有所增加；这可能与离子霉素和TPA脉冲后6小时IL - 2受体表达增加有关。这些结果表明，对于给定的T细胞克隆，Ca++依赖性蛋白激酶c的激活可以替代抗原受体触发事件，导致白细胞介素分泌和IL - 2受体表达增加，但不能替代IL - 2依赖性触发IL - 2受体。
A previous study indicated that Ca++ ionophores in conjunction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) could induce normal T lymphocytes to express receptors for the T cell growth factor, interleukin 2 (IL 2), to secrete IL 2, and to proliferate (1). Here we used long-term alloreactive Lyt-2+ cytotoxic or T4+ "helper" T cell clones. In response to their specific alloantigen, all of the clones secreted IFN-gamma but only the T4+ clone secreted IL 2 and proliferated in response to the appropriate alloantigen in the absence of exogenous IL 2. The Ca++ ionophore ionomycin and TPA, used in conjunction, mimicked the effect of specific alloantigen on these T cell clones, i.e., they induced the secretion of IFN-gamma in all clones and the secretion of IL 2 in the T4+ clone. In the absence of exogenous IL 2, a proliferative response was induced only for the IL 2 secreting clone. Increased sensitivity to exogenous IL 2 for some T cell clones was also observed after either alloantigen or ionomycin and TPA treatment; this could be correlated with an increase in the expression of IL 2 receptors 6 hr after a pulse with ionomycin and TPA. These results suggest that, for a given T cell clone, activation of the Ca++ -dependent protein kinase c can replace the antigen-receptor triggering events leading to interleukin secretion and increased expression of IL 2 receptors but cannot substitute for the IL 2 dependent triggering of the IL 2 receptor.