RNA-Seq Transcriptome Analysis in Date Palm Suggests Multi-Dimensional Responses to Salinity Stress
RNA-Seq Transcriptome Analysis in Date Palm Suggests Multi-Dimensional Responses to Salinity Stress
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DOI：
10.1007/s12042-015-9155-y
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发表时间：
2015-12-01
影响因子：
2
通讯作者：
Korban, Schuyler S.
中科院分区：
文献类型：
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作者：
Radwan, Osman;Arro, Jie;Korban, Schuyler S.
枣椰树在不利的非生物条件下生存的能力使其成为鉴定耐受基因的宝贵基因组资源。虽然耐盐机制已在模型和一些农作物中进行了大量研究，但尚未在枣椰树中进行此类研究。本研究的目的是通过转录组分析鉴定与应激耐受相关的基因，以支持进一步的功能研究。 Deglet Beida 品种的幼根经过盐胁迫处理，并用于 RNA-Seq 表达谱和透射电子显微镜 (TEM) 分析。使用 log2FC >= 15 发现总共 1939 个基因在模拟处理的根和盐胁迫根之间存在差异表达
The ability of date palm tree to survive under adverse abiotic conditions renders it as a valuable genomic resource for identifying tolerance genes. While mechanisms for salt tolerance have been heavily investigated in model as well as in some agronomic crops, no such studies have been undertaken in date palm. The aim of this study is to identify stress tolerance-related genes through transcriptomic analysis to support further functional studies. Young roots of Deglet Beida cultivar have been subjected to salt stress treatment, and used for RNA-Seq expression profiling and transmission electron microscopy (TEM) analysis. A total of 1939 genes are found to be differentially expressed between mock-treated roots and salt-stressed roots using log2FC >= 15