Cardioprotective and anti-oxidant effects of the terpenoid constituents of Ginkgo biloba extract (EGb 761)
Cardioprotective and anti-oxidant effects of the terpenoid constituents of Ginkgo biloba extract (EGb 761)
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DOI：
10.1006/jmcc.1996.0316
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发表时间：
1997-02-01
影响因子：
5
通讯作者：
Culcasi, M
中科院分区：
文献类型：
--
作者：
Pietri, S;Maurelli, E;Culcasi, M
血流动力学和电子自旋共振分析被用来评估在体内和体外的心脏保护和抗氧化作用的治疗相关剂量的银杏叶提取物（EGB 761）及其萜类成分（银杏内酯A和B，白果内酯）在大鼠。与安慰剂相比，EGb 761（60 mg/kg/天）和银杏内酯A（4 mg/kg/天）重复（15天）经口给药后观察到显著的抗缺血作用，表明心肌功能恢复改善。在银杏内酯A和B（均为0.05 μ g/ml）或白果内酯（0.15 μ g/ml），但不是Ebb 761（5 μ g/ml）存在下，对心脏进行体外缺血前和缺血后灌注，显著改善了所有血液动力学参数。缺血后冠状动脉流出物中5，5-二甲基-1-吡咯啉N-氧化物（DMPO）/羟基自由基自旋加合物（DMPG-OH）的水平在体内口服治疗或体外灌注EGb 761或萜烯后显著降低，最有效的化合物是银杏内酯A。由于萜烯的存在不影响超氧化物/DMPO加合物或DMPG-OH的形成，在超氧化物和羟基自由基发生器的非细胞试验中，其心脏保护作用似乎涉及抑制自由基形成，而不是直接清除自由基。(C)出版社：Academic Press Limited。
Hemodynamic and electron spin resonance analyses were used to assess the in vivo and in vitro cardioprotective and antioxidant effects of therapeutically relevant doses of Ginkgo biloba extract (EGb 761) and its terpenoid constituents (ginkgolides A and B, bilobalide) in the rat. Significant anti-ischemic effects, indicating improved myocardial functional recovery, were observed after repeated (15-day) oral treatments with both EGb 761 (60 mg/kg/day) and ginkgolide A (4 mg/kg/day), as compared to placebo. In vitro pre- and post-ischemic perfusion of hearts in the presence of the ginkgolides A and B (both at 0.05 mu g/ml) or bilobalide (0.15 mu g/ml), but not Ebb 761 (5 mu g/ml), significantly improved all hemodynamic parameters. Post-ischemic levels of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)/hydroxyl radical spin-adduct (DMPG-OH) in coronary effluents were Significantly decreased after in vivo oral treatments or after in vitro perfusion with EGb 761 or the terpenes, the most effective compound being ginkgolide A. As the presence of the terpenes did not influence the formation of the superoxide/DMPO adduct or DMPG-OH in acellular tests with superoxide and hydroxyl radical generators, their cardioprotective effects appear to involve an inhibition of free radical formation rather than direct free radical scavenging. (C) 1997 Academic Press Limited.