Benzoate Mediates Repression of C4-Dicarboxylate Utilization in “Aromatoleum aromaticum” EbN1
Benzoate Mediates Repression of C4-Dicarboxylate Utilization in “Aromatoleum aromaticum” EbN1
复制标题
苯甲酸盐介导“Aromatoleumaromaticum”EbN1 中 C4-二羧酸盐利用的抑制
DOI：
10.1128/jb.05072-11
复制
发表时间：
2011
影响因子：
3.2
通讯作者：
R. Rabus
中科院分区：
文献类型：
--
作者：
K. Trautwein;O. Grundmann;Lars Wöhlbrand;C. Eberlein;M. Boll;R. Rabus
摘要：在“Aromatoleum aromaticum”EbN1厌氧细胞中观察到双氧生长，因为从c4 -二羧酸盐（琥珀酸盐、富马酸盐或苹果酸盐）和苯甲酸盐的混合物中优先利用苯甲酸盐。差异蛋白谱（二维差异凝胶电泳[2D DIGE]）揭示了参与厌氧苯甲酸分解代谢和c4 -二羧酸盐摄取的蛋白质丰度的动态变化。在第一个活跃生长阶段，苯甲酸利用与厌氧苯甲酸降解相关的蛋白质（例如，苯甲酰辅酶A [CoA]还原酶）的最大丰度和DctP （EbA4158）的最小丰度相平行，DctP是预测的c4 -二羧酸三磷酸atp非依赖性周质（TRAP）转运体（DctPQM）的周质结合蛋白。在第二个生长期利用琥珀酸盐的过程中，观察到相反的情况。在苯甲酸盐消耗后，dctP（分别为dctPQM）转录物和dctP蛋白丰度的增加表明，抑制c4 -二羧酸盐摄取似乎是观察到的二羧酸的主要决定因素。
ABSTRACT Diauxic growth was observed in anaerobic C4-dicarboxylate-adapted cells of “Aromatoleum aromaticum” EbN1 due to preferred benzoate utilization from a substrate mixture of a C4-dicarboxylate (succinate, fumarate, or malate) and benzoate. Differential protein profiles (two-dimensional difference gel electrophoresis [2D DIGE]) revealed dynamic changes in abundance for proteins involved in anaerobic benzoate catabolism and C4-dicarboxylate uptake. In the first active growth phase, benzoate utilization was paralleled by maximal abundance of proteins involved in anaerobic benzoate degradation (e.g., benzoyl-coenzyme A [CoA] reductase) and minimal abundance of DctP (EbA4158), the periplasmic binding protein of a predicted C4-dicarboxylate tripartite ATP-independent periplasmic (TRAP) transporter (DctPQM). The opposite was observed during subsequent succinate utilization in the second active growth phase. The increased dctP (respectively, dctPQM) transcript and DctP protein abundance following benzoate depletion suggests that repression of C4-dicarboxylate uptake seems to be a main determinant for the observed diauxie.
影响因子：
7.4
作者：
Clauser, KR;Baker, P;Burlingame, AL
通讯作者：
Burlingame, AL
DOI：
10.1016/j.str.2009.08.011
发表时间：
2009-10-14
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Möglich A;Ayers RA;Moffat K
通讯作者：
Moffat K