Selecting representative microsatellite loci for genetic monitoring and analyzing genetic structure of an outbred population of orange tabby cats in China.
Selecting representative microsatellite loci for genetic monitoring and analyzing genetic structure of an outbred population of orange tabby cats in China.
复制标题
选取具有代表性的微卫星位点进行中国橙色虎斑猫远交群体的遗传监测和遗传结构分析。
DOI：
10.4238/2015.march.13.6
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Zhihai Chen
中科院分区：
文献类型：
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作者：
Xiaoyan Du;S. Yi;X. Huo;Chen Wang;D. Liu;Wen;Zhihai Chen
我们从猫和虎的遗传数据中优化了一组微卫星标记，用于有效的遗传监测，并使用它来分析中国远交猫种群的遗传结构。利用琼脂糖凝胶电泳技术，从131个猫微卫星位点和3个苏门答腊虎微卫星位点中筛选出一组多态性丰富的微卫星位点。然后，利用这组优化的遗传标记对中国一个远交橙子虎斑猫群体进行了简单串联重复序列分析。共筛选出31个多态性丰富的位点，单个位点的最高等位基因数为8个。对橙子虎斑猫群体的分析表明，平均观察到的等位基因数、平均有效等位基因数、平均Shannon信息指数、平均期望杂合度和观察杂合度分别为3.8387、2.4027、0.9787、0.5565和0.5528。所用的31个微卫星标记均具有多态性，适合于家猫的遗传结构分析。确认橙子虎斑猫种群是远交系种群。
We optimized a panel of microsatellite markers from cat and tiger genetic data for efficient genetic monitoring and used it to analyze the genetic structure of an outbred cat stock in China. We selected a set of rich polymorphic microsatellite loci from 131 cat microsatellite loci and 3 Sumatran tiger microsatellite loci using agarose gel electrophoresis. Next, the set of optimized genetic markers was used to analyze the genetic variation in an outbred population of orange tabby cats in China by simple-tandem repeat scanning. Thirty-one loci rich in polymorphisms were selected and the highest allele number in a single locus was 8. Analysis of the orange tabby cat population illustrated that the average observed number of alleles, mean effective allele number, mean Shannon's information index, mean expected heterozygosity, and observed heterozygosity were 3.8387, 2.4027, 0.9787, 0.5565, and 0.5528, respectively. The 31 microsatellite markers used were polymorphic and suitable for analyzing the genetic structure of cats. The population of orange tabby cats was confirmed to be a well-outbred stock.