Plant Peptides Govern Terminal Differentiation of Bacteria in Symbiosis
Plant Peptides Govern Terminal Differentiation of Bacteria in Symbiosis
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DOI：
10.1126/science.1184057
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发表时间：
2010-02-26
期刊：
影响因子：
56.9
通讯作者：
Mergaert, Peter
中科院分区：
文献类型：
--
作者：
Van de Velde, Willem;Zehirov, Grigor;Mergaert, Peter
豆科植物的根瘤中含有固氮内共生根瘤菌。在蒺藜苜蓿中，细菌经历由迄今未知的植物因子介导的不可逆（终末）分化。我们证明这些因子是结节特异性富含半胱氨酸（NCR）肽，它们靶向细菌并进入细菌膜和细胞质。 dnf1-1 信号肽酶突变体中 NCR 转运的阻碍与细菌终末分化的缺失相关。相反，在缺乏 NCR 的豆类中 NCR 的异位表达或用肽攻击培养的根瘤菌会引发终末分化的症状。由于 NCR 类似于抗菌肽，我们的研究结果揭示了宿主植物的一种先前未知的创新，它采用先天免疫系统的效应器进行共生，以操纵内共生细菌的细胞命运。
Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicago truncatula, the bacteria undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides that are targeted to the bacteria and enter the bacterial membrane and cytosol. Obstruction of NCR transport in the dnf1-1 signal peptidase mutant correlated with the absence of terminal bacterial differentiation. On the contrary, ectopic expression of NCRs in legumes devoid of NCRs or challenge of cultured rhizobia with peptides provoked symptoms of terminal differentiation. Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria.