Enhanced tendon healing with GDF 5 and 6
Enhanced tendon healing with GDF 5 and 6
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DOI：
10.3109/17453679909000958
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发表时间：
1999-02-01
期刊：
影响因子：
--
通讯作者：
Forslund, C
中科院分区：
文献类型：
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作者：
Aspenberg, P;Forslund, C
在断裂的肌腱末端，未分化的间充质细胞侵入血肿并分化形成再生肌腱。这种分化部分是由机械刺激引导的，这在临床上很难应用和控制。例如，跟腱断裂的闭合性治疗与再生肌腱再破裂的风险相关。外源性生长因子改善肌腱愈合以前没有报道。骨形态发生蛋白（Bone Morphogenetic Protein， BMP）家族中的3种蛋白——生长与分化因子（Growth and Differentiation Factors, GDFs） 5、6和7在大鼠肌内植入后诱导肌腱或韧带样组织，为改善肌腱加热提供了一种新的途径。我们对66只大鼠的跟腱进行了横切，并对小腿肌肉进行了去神经处理。去神经支配通过消除肌肉收缩来减少对肌腱痂的机械刺激。将GDF 5或GDF 6分两剂量植入胶原海绵，并与单独使用胶原海绵进行比较。2周后处死大鼠，发现这两种蛋白似乎以剂量依赖的方式增加了再生肌腱的拉伸强度。
Between ruptured tendon ends, undifferentiated mesenchymal cells invade the hematoma and differentiate to form a tendon regenerate. This differentiation is partly directed by mechanical stimuli, which are difficult to apply and control clinically. For example, closed treatment of Achilles tendon ruptures is associated with a risk of rerupture of the regenerate. Improved tendon healing by exogenous growth factors has not previously been reported. Three proteins in the Bone Morphogenetic Protein (BMP) family-namely Growth and Differentiation Factors (GDFs) 5, 6 and 7-have recently been shown to induce a tendon- or ligament-like tissue after intramuscular implantation in rats, indicating a new way to improve tendon heating. We transected the Achilles tendon in 66 rats and denervated the calf muscle. Denervation served to reduce the mechanical stimulation to the tendon callus by eliminating muscle contractions. GDF 5 or 6 were implanted on collagen sponges in the tendon defects in two doses and compared to collagen sponges alone. The rats were killed after 2 weeks and the tensile strength of the tendon regenerate was found to be increased by both proteins in a seemingly dose-dependent manner.