Developmental profile and hormonal regulation of the transcription factors broad and Kruppel homolog 1 in hemimetabolous thrips
Developmental profile and hormonal regulation of the transcription factors broad and Kruppel homolog 1 in hemimetabolous thrips
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DOI：
10.1016/j.ibmb.2010.11.004
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发表时间：
2011-02-01
影响因子：
3.8
通讯作者：
Tanaka, Toshiharu
中科院分区：
文献类型：
--
作者：
Minakuchi, Chieka;Tanaka, Miho;Tanaka, Toshiharu
在全变态昆虫中，Kruppel同源物1 （Kr-h1）和broad同源物（br）是幼体激素OH调控变态的关键参与者：Kr-h1是早期jh应答基因，br是指导蛹发育的转录因子。蓟马（Thysanoptera）被归类为半代谢昆虫，直接从若虫发育为成虫，但它们有静止和非摄食阶段，称为原蛹和蛹。分析了1个蛹期和1个蛹期的西部花蓟马（蓟科）和1个蛹期和2个蛹期（蛹I和蛹II）的br和Kr-h1基因的发育特征。在西花蓟马中，br mRNA水平在胚胎期中等，在幼虫-蛹期高，在幼虫-蛹期前和蛹期低，而Kr-h1 mRNA水平在胚胎期高，在幼虫和蛹期保持中等水平，在蛹期低。该基因在布氏螺旋体中的表达谱与在西方F中的表达谱非常相似，只是布氏螺旋体在幼虫末阶段表达的增加速度较慢，而在布氏螺旋体的蛹I中，品牌Kr-h1的mRNA水平保持在较高水平，然后下降。这些br和Kr-h1的谱图与全代谢昆虫的相似，尽管在蓟马胚胎发育中发现的br表达让人想起一些半代谢物种。在不同的发育阶段用外源性JH模拟物（OHM）处理，一致导致西方F.或短叶H. II蛹的致命性。JHM处理新蜕皮的蜂蛹后，两种蜂蛹中Kr-h1和br的表达均延长，表明Kr-h1和br可能参与了JHM抗变质信号的介导。(C) 2010 Elsevier Ltd.版权所有。
In holometabolous insects, Kruppel homolog 1 (Kr-h1) and broad (br) are key players in the juvenile hormone OH) regulation of metamorphosis: Kr-h1 is an early JH-response gene, while br is a transcription factor that directs pupal development. Thrips (Thysanoptera) are classified as hemimetabolous insects that develop directly from nymph to adult, but they have quiescent and non-feeding stages called propupa and pupa. We analyzed the developmental profiles of br and Kr-h1 in the western flower thrips Frankliniella occidentalis (Thripidae) that has one propupal instar and one pupal instar, and Haplothrips brevitubus (Phlaeothripidae) that has one propupal instar and two pupal instars, i.e. pupa I and pupa II. In E occidentalis, the br mRNA levels were moderate in the embryonic stage, high at the larva-propupa transition, and low in the pre-final larval instar and the pupal stage, while Kr-h1 mRNA levels were high in the embryonic stage, remained at a moderate level in the larval and propupal stages, and low in the pupal stage. The expression profiles in H. brevitubus were very similar to those in F occidentalis, except that the increase of br expression in the final larval stage occurs more slowly in H. brevitubus, and that the mRNA levels of brand Kr-h1 remained high in pupa I of H. brevitubus and then decreased. These profiles of br and Kr-h1 were comparable to those in holometabolous insects, although br expression found in thrips' embryogenesis is reminiscent of several hemimetabolous species. Treatment with an exogenous JH mimic OHM) in distinct developmental stages consistently resulted in lethality as pupa of F. occidentalis or pupa II of H. brevitubus. Treatment with JHM to newly molted propupae caused prolonged expression of Kr-h1 and br in both species, suggesting that Kr-h1 and br could be involved in mediating anti-metamorphic signals of JHM. (C) 2010 Elsevier Ltd. All rights reserved.