Decreased in vivo binding to brain benzodiazepine receptors during social isolation
Decreased in vivo binding to brain benzodiazepine receptors during social isolation
复制标题
社会隔离期间体内与大脑苯二氮卓受体的结合减少
DOI：
10.1007/bf00442234
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发表时间：
2004
影响因子：
3.4
通讯作者：
T. Insel
中科院分区：
文献类型：
--
作者：
T. Insel
药理学研究表明，苯二氮卓类药物可以调节与幼鼠社交分离相关的超声波发声 (USV)。在这项研究中，使用体内受体放射自显影术来确定大脑苯二氮卓受体在社会分离期间是否在功能上较难结合外源配体。在 25 分钟的社会隔离期间，将标记的苯二氮卓受体拮抗剂 3H-RO 15-1788 给予 10 日龄的幼鼠。在对分离幼崽的大脑进行放射自显影研究中，新皮质（额叶、运动和体感）、海马以及上丘和下丘中 3 H-RO 15-1788 的结合减少。由于这些相同区域未显示 3 H-RO 15-1788 的体外结合发生改变，因此这些体内结合减少并不反映受体数量的变化。讨论了体内结合减少的解释以及这些结果对定义分离行为的神经基质的影响。
Pharmacologic studies have demonstrated that benzodiazepines can modulate the ultrasonic vocalizations (USV) associated with social separation of rat pups. In this study, in vivo receptor autoradiography was used to determine if brain benzodiazepine receptors were functionally less available to bind an exogenous ligand during social separation. The labelled benzodiazepine receptor antagonist3H-RO 15-1788 was given to 10-day-old rat pups during 25minutes of social separation. In autoradiographic studies of brains from separated pups, the binding of3H-RO 15-1788 was decreased in neocortex (frontal, motor, and somatosensory), hippocampus, and superior and inferior colliculi. As these same regions showed no alteration of in vitro binding of3H-RO 15-1788, these in vivo binding decreases do not reflect changes in receptor number. The interpretation of decreased in vivo binding and implications of these results for defining the neural substrates of separation behavior are discussed.