Ionic-Liquid-Doped Polyaniline Inverse Opals: Preparation, Characterization, and Application for the Electrochemical Impedance Immunoassay of Hepatitis B Surface Antigen
Ionic-Liquid-Doped Polyaniline Inverse Opals: Preparation, Characterization, and Application for the Electrochemical Impedance Immunoassay of Hepatitis B Surface Antigen
复制标题
离子液体掺杂聚苯胺反蛋白石：制备、表征及其在乙型肝炎表面抗原电化学阻抗免疫分析中的应用
DOI：
10.1002/adfm.200901003
复制
发表时间：
2009-10-09
影响因子：
19
通讯作者：
Zhu, Jun-Jie
中科院分区：
文献类型：
--
作者：
Li, Xing-Hua;Dai, Lin;Zhu, Jun-Jie
采用电聚合法制备了3D有序大孔离子液体掺杂聚苯胺（IL-PANI）反蛋白石薄膜，并通过静电吸附法将金纳米粒子组装在该薄膜上.该膜可用于制备电化学阻抗谱（EIS）免疫传感器，用于检测B表面抗原（HBsAg）。使用EIS技术，通过监测HBsAg和HBsAg Ab（表面抗体）之间的相应特异性结合来测量HBsAg的浓度。增加的电子转移电阻（R-et）值与HBsAg浓度的对数值成正比。该方法的线性范围为0.032 pg/mL ~ 31.6 ng/mL，检出限为0.001 pg/mL。h使用者
A 3D ordered macroporous (3DOM) ionic-liquid-doped polyaniline (IL-PANI) inverse opaline film is fabricated with an electropolymerization method and gold nanoparticles; (AuNPs) are assembled on the film by electrostatic adsorption, which offers a promising basis for biomolecular immobilization due to its satisfactory chemical stability, good electronic conductivity, and excellent biocompatibility. The AuNP/IL-PANI inverse opaline film could be used to fabricate an electrochemical impedance spectroscopy (EIS) immunosensor for the determination of Hepatitis B surface antigen (HBsAg). The concentration of HBsAg is measured using the EIS technique by monitoring the corresponding specific binding between HBsAg and HBsAb (surface antibody). The increased electron transfer resistance (R-et) values are proportional to the logarithmic value of the concentration of HBsAg. This novel immunoassay displays a linear response range between 0.032 pg mL(-1) and 31 6 nor mL(-1) with a detection limit of 0.001 pg mL(-1) The detection of HBsAg levels in several sera showed satisfactory agreement wit a commercial turbidimetric method. h those using