Biodegradation of 2-bromonitrobenzene by Pseudomonas stutzeri ZWLR2-1
Biodegradation of 2-bromonitrobenzene by Pseudomonas stutzeri ZWLR2-1
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施氏假单胞菌 ZWLR2-1 对 2-溴硝基苯的生物降解
DOI：
10.1016/j.ibiod.2018.12.008
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ning-Yi Zhou
中科院分区：
文献类型：
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作者：
Lei Wang;Yi-Zhou Gao;Huan Zhao;Ying Xu;Ning-Yi Zhou
卤代硝基苯对人类健康和环境危害极大。其中，有报道称，氯代硝基苯可被几种细菌菌株降解。然而，到目前为止，还没有任何纯细菌培养物的溴硝基苯生物降解的报道。在这项研究中，2-溴硝基苯被2-氯硝基苯利用菌施氏假单胞菌ZWLR 2 -1利用作为生长的唯一碳源、氮源和能量源，并释放亚硝酸盐。来自菌株ZWLR 2 -1的硝基芳香族双加氧酶CnbAaAbAcAd将2-溴硝基苯转化为3-溴邻苯二酚，同时释放亚硝酸盐。CnbC具有3-溴邻苯二酚1，2-双加氧酶活性。通过液相色谱-质谱仪（LC-MS）与真实的3-溴邻苯二酚进行比较，确认了3-溴邻苯二酚的身份。这些结果表明，Stutzeri假单胞菌ZWLR 2 -1的NB簇负责2-氯硝基苯和2-溴硝基苯的生物降解，提供了另一个细菌快速进化利用多种外源性物质的途径的例子。
Halonitrobenzenes are highly harmful to human health and the environment. Among them, chloronitrobenzenes have been reported to be degraded by several bacterial strains. However, till date, the biodegradation of bromonitrobenzenes has not been reported for any pure bacterial culture. In this study, 2-bromonitrobenzene was found to be utilized by the 2-chloronitrobenzene utilizerPseudomonas stutzeriZWLR2-1 as the sole source of carbon, nitrogen and energy for growth, with nitrite release. The nitroaromatic dioxygenase CnbAaAbAcAd from strain ZWLR2-1 converted 2-bromonitrobenzene to 3-bromocatechol with concomitant nitrite release. CnbC exhibited 3-bromocatechol 1,2-dioxygenase activity. The identity of 3-bromocatechol was confirmed by Liquid Chromatograph-Mass Spectrometer (LC-MS), in comparison with authentic 3-bromocatechol. These results demonstrated that thecnbcluster ofPseudomonas stutzeriZWLR2-1 is responsible for the biodegradation of both 2-chloronitrobenzene and 2-bromonitrobenzene, providing yet another example of a bacterium having rapidly evolved a pathway to utilize multiple xenobiotics.