Oxygen-dependent reperfusion injury in the isolated rat lung.
Oxygen-dependent reperfusion injury in the isolated rat lung.
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离体大鼠肺的氧依赖性再灌注损伤。
DOI：
10.1152/jappl.1992.72.4.1454
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Fisher,AB
中科院分区：
文献类型：
--
作者：
Eckenhoff,RG;Dodia,C;Tan,Z;Fisher,AB
为了进一步明确肺缺血损伤的氧依赖性与缺血再灌注的关系，我们采用了大鼠离体肺灌流通气模型，将氧合和灌流分离。在缺血期间，肺通气与各种氧气浓度，然后在60分钟的再灌注期间与95%的氧气通气。其他肺在缺血期间用0%氧（氮）通气，并且再灌注相氧浓度是变化的。组织和灌流液脂质过氧化产物（硫代巴比妥酸反应物质和共轭二烯），干/湿重比，和乳酸脱氢酶作为肺损伤的指标进行了测定。此外，还对一些肺进行了电子显微镜检查。结果表明，在缺血和再灌注阶段的脂质过氧化的氧依赖性，但脂质过氧化是几倍更大的再灌注比缺血阶段。脂质过氧化产物与肺损伤指标（干湿重比、乳酸脱氢酶和电镜）密切相关。
To further define the relationship between oxygen dependence of lung injury during ischemia and ischemia-reperfusion, we used the isolated, perfused, and ventilated rat lung model, so that oxygenation and perfusion could be separated. During ischemia, lungs were ventilated with various oxygen concentrations and then ventilated with 95% oxygen during the 60-min reperfusion period. Other lungs were ventilated with 0% oxygen (nitrogen) during ischemia, and the reperfusion phase oxygen concentration was varied. Tissue and perfusate lipid peroxidation products (thiobarbituric acid-reactive substances and conjugated dienes), dry-to-wet weight ratio, and lactate dehydrogenase were measured as indexes of lung damage. In addition, electron microscopy of some lungs was performed. Results demonstrate an oxygen dependence of lipid peroxidation in both the ischemic and reperfusion phases, but lipid peroxidation is severalfold greater in the reperfusion than in the ischemic phase. Products of lipid peroxidation closely correlate with indexes of lung injury (dry-to-wet weight ratio, lactate dehydrogenase, and electron microscopy).