A SENSITIVE IMMUNOASSAY FOR PORCINE INTERFERON-ALPHA
A SENSITIVE IMMUNOASSAY FOR PORCINE INTERFERON-ALPHA
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DOI：
10.1016/0165-2427(92)90102-v
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发表时间：
1992-01-31
影响因子：
1.8
通讯作者：
ALM, GV
中科院分区：
文献类型：
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作者：
DEARCE, HD;ARTURSSON, K;ALM, GV
选择两种针对重组猪干扰素α (ifn - α)不同表位的小鼠单克隆抗体（mab），构建双位点ELISA。该酶联免疫吸附试验一步法检测到约0.5单位ml-1的ifn - α，灵敏度与细胞病变效应抑制生物测定法相似，但精度更高。两种方法对组织培养基中ifn - α的估计相关性较好。相反，猪血清中的一个或几个因素降低了ELISA的敏感性。然而，猪血清中ifn - α的测量可以通过ELISA的两步版本进行，其灵敏度约为1单位ifn - α ml-1。ELISA和生物测定的结果一致，除了ELISA可能在总共91个阴性的scra中产生假阳性结果。另外，23例阳性血清中有1例ELISA阴性。
Two murine monoclonal antibodies (mAbs) directed against different epitopes on recombinant porcine interferon-alpha (IFN-alpha) were selected and used to construct a two-site ELISA. This ELISA, when performed in a one-step version, detected about 0.5 units ml-1 of IFN-alpha and showed similar sensitivity but better precision than a cytopathic effect inhibition bioassay. Estimates of IFN-alpha in tissue culture medium by the two assays correlated well. In contrast, one or several factors in porcine serum reduced the sensitivity of the ELISA. Measurements of IFN-alpha in porcine serum was, however, possible in a two-step version of the ELISA, with a sensitivity of about 1 unit IFN-alpha ml-1. Results of ELISA and bioassay agreed, except that the ELISA possibly produced false positive results in two out of a total of 91 scra negative in the bioassay. In addition, one of 23 sera positive in the bioassay was negative in the ELISA.